Inhibition of rat mixed lymphocyte pancreatic islet cultures with anti-Ia immunotoxin

Judith A. Shizuru, Sundaram Ramakrishnan, Thurman Hunt, Ronald G. Merrell, C. Garrison Fathman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Studies reported here indicate that an anti-Ia immunotoxin can eliminate the allostimulatory subpopulation of cells present within the islets of Langerhans without damaging the hormone-secreting cells. Such studies made use of an in vitro correlate of transplantation rejection, the mixed lymphocyte islet cell (MLIC) reaction. Using the MLIC, it was demonstrated that an anti-Ia immunotoxin removed cells capable of stimulating the MLIC in a dose-dependent fashion without altering the hormone-secreting functions of the remaining cells when challenged with glucose and theophylline. These studies suggest the feasibility of using such anti-Ia immunotoxins in islet allograft transplantation models to circumvent problems inherent in complement-mediated cytotoxicity, a previously documented effective form of inducing islet allotransplantation tolerance.

Original languageEnglish (US)
Pages (from-to)660-666
Number of pages7
JournalTransplantation
Volume42
Issue number6
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Immunotoxins
Islets of Langerhans
Lymphocytes
Hormones
Islets of Langerhans Transplantation
Graft Rejection
Feasibility Studies
Theophylline
Allografts
Glucose

ASJC Scopus subject areas

  • Transplantation

Cite this

Inhibition of rat mixed lymphocyte pancreatic islet cultures with anti-Ia immunotoxin. / Shizuru, Judith A.; Ramakrishnan, Sundaram; Hunt, Thurman; Merrell, Ronald G.; Fathman, C. Garrison.

In: Transplantation, Vol. 42, No. 6, 01.01.1986, p. 660-666.

Research output: Contribution to journalArticle

Shizuru, Judith A. ; Ramakrishnan, Sundaram ; Hunt, Thurman ; Merrell, Ronald G. ; Fathman, C. Garrison. / Inhibition of rat mixed lymphocyte pancreatic islet cultures with anti-Ia immunotoxin. In: Transplantation. 1986 ; Vol. 42, No. 6. pp. 660-666.
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