Abstract
As a model for the interaction between macrophages and mucus in the tracheobronchial tree, we incubated sheep alveolar macrophages in vitro and without airway mucus from the same animal and tested their phagocytic function and rates of general protein synthesis. Airway mucus was obtained by tracheal suction after previous subcutaneous injection of the sheep with pilocarpine (0.5 mg/kg) and subsequently diluted. Pulmonary macrophages were obtained by saline lavage through a balloon-tipped fiber-optic bronchoscope. In incubated attached macrophages, phenylalanine incorporation into protein was inhibited 51% by mucus (P <0.001). Phagocytosis of 1.09-μm latex particles was inhibited 75% by mucus at non-saturating levels of particles (P <0.001) and by 47% at saturating levels of particles (P <0.001). Inhibitory activity for protein synthesis and phagocytosis was retained in the 104,000 g supernatant fraction of whole mucus (sol), while the gel fraction inhibited phagocytosis only. This difference did not appear to be related to physical factors. We conclude that diluted airway mucus interferes with pulmonary macrophage function as assessed by rates of general protein synthesis and phagocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 94-98 |
| Number of pages | 5 |
| Journal | Journal of Applied Physiology Respiratory Environmental and Exercise Physiology |
| Volume | 54 |
| Issue number | 1 |
| State | Published - May 26 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
- Endocrinology
Cite this
Inhibition of pulmonary macrophage function by airway mucus. / Woodside, K. H.; Denas, S. M.; Smith, K. L.; Kim, C. S.; Wanner, Adam.
In: Journal of Applied Physiology Respiratory Environmental and Exercise Physiology, Vol. 54, No. 1, 26.05.1983, p. 94-98.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of pulmonary macrophage function by airway mucus
AU - Woodside, K. H.
AU - Denas, S. M.
AU - Smith, K. L.
AU - Kim, C. S.
AU - Wanner, Adam
PY - 1983/5/26
Y1 - 1983/5/26
N2 - As a model for the interaction between macrophages and mucus in the tracheobronchial tree, we incubated sheep alveolar macrophages in vitro and without airway mucus from the same animal and tested their phagocytic function and rates of general protein synthesis. Airway mucus was obtained by tracheal suction after previous subcutaneous injection of the sheep with pilocarpine (0.5 mg/kg) and subsequently diluted. Pulmonary macrophages were obtained by saline lavage through a balloon-tipped fiber-optic bronchoscope. In incubated attached macrophages, phenylalanine incorporation into protein was inhibited 51% by mucus (P <0.001). Phagocytosis of 1.09-μm latex particles was inhibited 75% by mucus at non-saturating levels of particles (P <0.001) and by 47% at saturating levels of particles (P <0.001). Inhibitory activity for protein synthesis and phagocytosis was retained in the 104,000 g supernatant fraction of whole mucus (sol), while the gel fraction inhibited phagocytosis only. This difference did not appear to be related to physical factors. We conclude that diluted airway mucus interferes with pulmonary macrophage function as assessed by rates of general protein synthesis and phagocytosis.
AB - As a model for the interaction between macrophages and mucus in the tracheobronchial tree, we incubated sheep alveolar macrophages in vitro and without airway mucus from the same animal and tested their phagocytic function and rates of general protein synthesis. Airway mucus was obtained by tracheal suction after previous subcutaneous injection of the sheep with pilocarpine (0.5 mg/kg) and subsequently diluted. Pulmonary macrophages were obtained by saline lavage through a balloon-tipped fiber-optic bronchoscope. In incubated attached macrophages, phenylalanine incorporation into protein was inhibited 51% by mucus (P <0.001). Phagocytosis of 1.09-μm latex particles was inhibited 75% by mucus at non-saturating levels of particles (P <0.001) and by 47% at saturating levels of particles (P <0.001). Inhibitory activity for protein synthesis and phagocytosis was retained in the 104,000 g supernatant fraction of whole mucus (sol), while the gel fraction inhibited phagocytosis only. This difference did not appear to be related to physical factors. We conclude that diluted airway mucus interferes with pulmonary macrophage function as assessed by rates of general protein synthesis and phagocytosis.
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M3 - Article
VL - 54
SP - 94
EP - 98
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 1
ER -