Inhibition of NFkB DNA binding activity by glucocorticoids in rat brain

Tino Unlap, Richard S. Jope

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The influence of glucocorticoids on the transcription factor NFkB was investigated by using the gel mobility shift assay with nuclear extracts prepared from rat cerebral cortex and hippocampus after a variety of in vivo treatments. Following stimulation with each of three treatments, kainate, pilocarpine, or lithium plus pilocarpine-induced seizures, NFkB DNA binding activity was significantly greater in the cortex and hippocampus from adrenalectomized than from adrenal-intact rats. These results indicate that in rat brain glucocorticoids inhibit NFkB activity in addition to the previously reported inhibition of the transcription factor AP-1 (activator protein 1). Impairment of stimulus-induced transcription factor activity may contribute to the deleterious effects of prolonged elevations of glucocorticoids on neuronal function.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalNeuroscience Letters
Volume198
Issue number1
DOIs
StatePublished - Sep 22 1995
Externally publishedYes

Keywords

  • Glucocorticoids
  • Kainate
  • NFkB
  • Pilocarpine
  • Seizures
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)

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