Inhibition of MSH Release in Frogs by Direct Application of L-Prolyl-L-leucylglycinamide to the Pituitary

Abba J. Kastin, Andrew V. Schally, Sharon Viosca

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Ten nanograms of L-prolyl-L-leucylglycinamide (PLG), when applied directly to the pituitary, resulted in lightening of a frog whose skin was previously darkened by destruction of the hypothalamus. As much as 10 mg of PLG was inactive when injected into the dorsal lymph sac or aortic trunk. Incubation of PLG for 10 min in frog serum caused significant inactivation of the material. This inactivation may partially account for the poor MIF activity observed after systemic injection of PLG. The newly modified assay described here is particularly recommended for future studies with MIF because of its simplicity.

Original languageEnglish (US)
Pages (from-to)1437-1439
Number of pages3
JournalProceedings of the Society for Experimental Biology and Medicine
Volume137
Issue number4
DOIs
StatePublished - Sep 1971
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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