Inhibition of microRNA suppression of dishevelled results in Wnt pathway-associated developmental defects in sea urchin

Nina Faye Sampilo, Nadezda A. Stepicheva, Syed Aun Murtaza Zaidi, Lingyu Wang, Wei Wu, Athula Wikramanayake, Jia L. Song

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

MicroRNAs (miRNAs) are highly conserved, small non-coding RNAs that regulate gene expressions by binding to the 3′ untranslated region of target mRNAs thereby silencing translation. Some miRNAs are key regulators of the Wnt signaling pathways, which impact developmental processes. This study investigates miRNA regulation of different isoforms of Dishevelled (Dvl/Dsh), which encode a key component in the Wnt signaling pathway. The sea urchin Dvl mRNA isoforms have similar spatial distribution in early development, but one isoform is distinctively expressed in the larval ciliary band. We demonstrated that Dvl isoforms are directly suppressed by miRNAs. By blocking miRNA suppression of Dvl isoforms, we observed dosedependent defects in spicule length, patterning of the primary mesenchyme cells, gut morphology, and cilia. These defects likely result from increased Dvl protein levels, leading to perturbation of Wnt-dependent signaling pathways and additional Dvl-mediated processes. We further demonstrated that overexpression of Dvl isoforms recapitulated some of the Dvl miRNATP-induced phenotypes. Overall, our results indicate that miRNA suppression of Dvl isoforms plays an important role in ensuring proper development and function of primary mesenchyme cells and cilia.

Original languageEnglish (US)
Article numberdev167130
JournalDevelopment (Cambridge)
Volume145
Issue number23
DOIs
StatePublished - Dec 1 2018

Fingerprint

Wnt Signaling Pathway
Sea Urchins
MicroRNAs
Protein Isoforms
Cilia
Mesoderm
RNA Isoforms
Small Untranslated RNA
3' Untranslated Regions
Phenotype
Gene Expression
Messenger RNA
Proteins

Keywords

  • Cilia
  • MiRNA target protector
  • Post-transcriptional regulation
  • Primary mesenchyme cells
  • Sea urchin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Inhibition of microRNA suppression of dishevelled results in Wnt pathway-associated developmental defects in sea urchin. / Sampilo, Nina Faye; Stepicheva, Nadezda A.; Zaidi, Syed Aun Murtaza; Wang, Lingyu; Wu, Wei; Wikramanayake, Athula; Song, Jia L.

In: Development (Cambridge), Vol. 145, No. 23, dev167130, 01.12.2018.

Research output: Contribution to journalArticle

Sampilo, Nina Faye ; Stepicheva, Nadezda A. ; Zaidi, Syed Aun Murtaza ; Wang, Lingyu ; Wu, Wei ; Wikramanayake, Athula ; Song, Jia L. / Inhibition of microRNA suppression of dishevelled results in Wnt pathway-associated developmental defects in sea urchin. In: Development (Cambridge). 2018 ; Vol. 145, No. 23.
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