Inhibition of lung cancer growth in mice by dietary mixed tocopherols

Joshua D. Lambert, Gang Lu, Mao Jung Lee, Jennifer Hu, Jihyeung Ju, Chung S. Yang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Tocopherols are lipophilic antioxidants found in vegetable oils. Here, we examined the growth inhibitory effect of a γ-tocopherol-enriched tocopherol mixture (γTmT) against CL13 murine lung cancer cells grown in culture and as subcutaneous tumors in A/J mice. We found γTmT had no effect after 2 days and weakly inhibited the growth of CL13 in culture after 5 days (28% growth inhibition at 80 μM). Dietary treatment with 0.1 and 0.3% γTmT for 50 days inhibited the growth of CL13 tumors in A/J mice by 53.9 and 80.5%, respectively. Histopathological analysis revealed an increase in tumor necrosis compared to control tumors (80 and 240% increase by 0.1 and 0.3% γTmT, respectively). Dietary treatment with γTmT dose-dependently increased γ- (10.0-37.6-fold) and δ-tocopherol (8.9-26.7-fold) in the tumors of treated mice compared to controls. Dietary treatment with γTmT also increased plasma γ- (5.4-6.7-fold) and δ-tocopherol (5.5-7-fold). Whereas others have demonstrated the cancer preventive activity of γTmT against mammary and colon cancer, this is the first report of growth inhibitory activity against lung cancer. Further studies are needed to determine the underlying mechanisms for this anticancer activity, and to determine if such activity occurs in other models of cancer.

Original languageEnglish
Pages (from-to)1030-1035
Number of pages6
JournalMolecular Nutrition and Food Research
Volume53
Issue number8
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

Fingerprint

Tocopherols
lung neoplasms
tocopherols
Lung Neoplasms
mice
Growth
neoplasms
Neoplasms
Plant Oils
colorectal neoplasms
vegetable oil
Colonic Neoplasms
growth retardation
breasts
necrosis
Necrosis
Antioxidants
Breast Neoplasms
antioxidants

Keywords

  • 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone
  • Lung cancer
  • Syngeneic tumor model
  • Tocopherols
  • Tumor necrosis

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Inhibition of lung cancer growth in mice by dietary mixed tocopherols. / Lambert, Joshua D.; Lu, Gang; Lee, Mao Jung; Hu, Jennifer; Ju, Jihyeung; Yang, Chung S.

In: Molecular Nutrition and Food Research, Vol. 53, No. 8, 01.08.2009, p. 1030-1035.

Research output: Contribution to journalArticle

Lambert, Joshua D. ; Lu, Gang ; Lee, Mao Jung ; Hu, Jennifer ; Ju, Jihyeung ; Yang, Chung S. / Inhibition of lung cancer growth in mice by dietary mixed tocopherols. In: Molecular Nutrition and Food Research. 2009 ; Vol. 53, No. 8. pp. 1030-1035.
@article{88eb8e62ed0b42989160f4e36db4eefa,
title = "Inhibition of lung cancer growth in mice by dietary mixed tocopherols",
abstract = "Tocopherols are lipophilic antioxidants found in vegetable oils. Here, we examined the growth inhibitory effect of a γ-tocopherol-enriched tocopherol mixture (γTmT) against CL13 murine lung cancer cells grown in culture and as subcutaneous tumors in A/J mice. We found γTmT had no effect after 2 days and weakly inhibited the growth of CL13 in culture after 5 days (28{\%} growth inhibition at 80 μM). Dietary treatment with 0.1 and 0.3{\%} γTmT for 50 days inhibited the growth of CL13 tumors in A/J mice by 53.9 and 80.5{\%}, respectively. Histopathological analysis revealed an increase in tumor necrosis compared to control tumors (80 and 240{\%} increase by 0.1 and 0.3{\%} γTmT, respectively). Dietary treatment with γTmT dose-dependently increased γ- (10.0-37.6-fold) and δ-tocopherol (8.9-26.7-fold) in the tumors of treated mice compared to controls. Dietary treatment with γTmT also increased plasma γ- (5.4-6.7-fold) and δ-tocopherol (5.5-7-fold). Whereas others have demonstrated the cancer preventive activity of γTmT against mammary and colon cancer, this is the first report of growth inhibitory activity against lung cancer. Further studies are needed to determine the underlying mechanisms for this anticancer activity, and to determine if such activity occurs in other models of cancer.",
keywords = "4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone, Lung cancer, Syngeneic tumor model, Tocopherols, Tumor necrosis",
author = "Lambert, {Joshua D.} and Gang Lu and Lee, {Mao Jung} and Jennifer Hu and Jihyeung Ju and Yang, {Chung S.}",
year = "2009",
month = "8",
day = "1",
doi = "10.1002/mnfr.200800438",
language = "English",
volume = "53",
pages = "1030--1035",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "8",

}

TY - JOUR

T1 - Inhibition of lung cancer growth in mice by dietary mixed tocopherols

AU - Lambert, Joshua D.

AU - Lu, Gang

AU - Lee, Mao Jung

AU - Hu, Jennifer

AU - Ju, Jihyeung

AU - Yang, Chung S.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Tocopherols are lipophilic antioxidants found in vegetable oils. Here, we examined the growth inhibitory effect of a γ-tocopherol-enriched tocopherol mixture (γTmT) against CL13 murine lung cancer cells grown in culture and as subcutaneous tumors in A/J mice. We found γTmT had no effect after 2 days and weakly inhibited the growth of CL13 in culture after 5 days (28% growth inhibition at 80 μM). Dietary treatment with 0.1 and 0.3% γTmT for 50 days inhibited the growth of CL13 tumors in A/J mice by 53.9 and 80.5%, respectively. Histopathological analysis revealed an increase in tumor necrosis compared to control tumors (80 and 240% increase by 0.1 and 0.3% γTmT, respectively). Dietary treatment with γTmT dose-dependently increased γ- (10.0-37.6-fold) and δ-tocopherol (8.9-26.7-fold) in the tumors of treated mice compared to controls. Dietary treatment with γTmT also increased plasma γ- (5.4-6.7-fold) and δ-tocopherol (5.5-7-fold). Whereas others have demonstrated the cancer preventive activity of γTmT against mammary and colon cancer, this is the first report of growth inhibitory activity against lung cancer. Further studies are needed to determine the underlying mechanisms for this anticancer activity, and to determine if such activity occurs in other models of cancer.

AB - Tocopherols are lipophilic antioxidants found in vegetable oils. Here, we examined the growth inhibitory effect of a γ-tocopherol-enriched tocopherol mixture (γTmT) against CL13 murine lung cancer cells grown in culture and as subcutaneous tumors in A/J mice. We found γTmT had no effect after 2 days and weakly inhibited the growth of CL13 in culture after 5 days (28% growth inhibition at 80 μM). Dietary treatment with 0.1 and 0.3% γTmT for 50 days inhibited the growth of CL13 tumors in A/J mice by 53.9 and 80.5%, respectively. Histopathological analysis revealed an increase in tumor necrosis compared to control tumors (80 and 240% increase by 0.1 and 0.3% γTmT, respectively). Dietary treatment with γTmT dose-dependently increased γ- (10.0-37.6-fold) and δ-tocopherol (8.9-26.7-fold) in the tumors of treated mice compared to controls. Dietary treatment with γTmT also increased plasma γ- (5.4-6.7-fold) and δ-tocopherol (5.5-7-fold). Whereas others have demonstrated the cancer preventive activity of γTmT against mammary and colon cancer, this is the first report of growth inhibitory activity against lung cancer. Further studies are needed to determine the underlying mechanisms for this anticancer activity, and to determine if such activity occurs in other models of cancer.

KW - 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone

KW - Lung cancer

KW - Syngeneic tumor model

KW - Tocopherols

KW - Tumor necrosis

UR - http://www.scopus.com/inward/record.url?scp=69149085802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69149085802&partnerID=8YFLogxK

U2 - 10.1002/mnfr.200800438

DO - 10.1002/mnfr.200800438

M3 - Article

C2 - 19557822

AN - SCOPUS:69149085802

VL - 53

SP - 1030

EP - 1035

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 8

ER -