Abstract
Antibody against synthetic LHRH was generated in ewes by repeated intradermal injection with Glu(I)-LHRH coupled with human serum albumin and then separated anti-LHRH gamma globulin (A-LHRHγG) by ammonium sulphate precipitation technique. Daily injection of A-LHRHγG from day 1 through day 7 (1ml,iv), caused delayed implantation for 7-8 days and only 2 in 5 rats could maintain normal pregnancy course until term. Control rats in which normal sheep gamma globulin (NSG) was injected under the same protocol, could undergo normal fetal development. Daily injection of A-LHRHγG from day 3 through day 5 delayed the nidation process for 4-6 days. Plasma estrogen in the A-LHRHγG treated rats was maintained at low levels, and raised significantly on day 4 and 5 in NSG treated control rats. A single injection of 1 ml of A-LHRHγG on day 4 inhibited implantation on day 8, but injection on day 3 or 5 did not. Delayed implantation by A-LHRHγG injected on day 4 was overcome by concomitant administration of 2 subcutaneous injections of Iug of synthetic LHRH, or single subcutaneous injection of Iug of E2. The results indicate that the hypothalamic LHRH is essential on day 4 of pregnancy for timely implantation of fertilized ova, presumably by maintaining LH and consequently estrogen secretion from ovaries.
| Original language | English |
|---|---|
| Pages (from-to) | 1717-1723 |
| Number of pages | 7 |
| Journal | Acta Obstetrica et Gynaecologica Japonica |
| Volume | 29 |
| Issue number | 12 |
| State | Published - Dec 1 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Obstetrics and Gynecology
Cite this
Inhibition of implantation by the administration of anti-LHRH immunogamma globulin in rats. / Nishi, N.; Saito, M.; Arimura, A.; Schally, Andrew V.
In: Acta Obstetrica et Gynaecologica Japonica, Vol. 29, No. 12, 01.12.1977, p. 1717-1723.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of implantation by the administration of anti-LHRH immunogamma globulin in rats
AU - Nishi, N.
AU - Saito, M.
AU - Arimura, A.
AU - Schally, Andrew V
PY - 1977/12/1
Y1 - 1977/12/1
N2 - Antibody against synthetic LHRH was generated in ewes by repeated intradermal injection with Glu(I)-LHRH coupled with human serum albumin and then separated anti-LHRH gamma globulin (A-LHRHγG) by ammonium sulphate precipitation technique. Daily injection of A-LHRHγG from day 1 through day 7 (1ml,iv), caused delayed implantation for 7-8 days and only 2 in 5 rats could maintain normal pregnancy course until term. Control rats in which normal sheep gamma globulin (NSG) was injected under the same protocol, could undergo normal fetal development. Daily injection of A-LHRHγG from day 3 through day 5 delayed the nidation process for 4-6 days. Plasma estrogen in the A-LHRHγG treated rats was maintained at low levels, and raised significantly on day 4 and 5 in NSG treated control rats. A single injection of 1 ml of A-LHRHγG on day 4 inhibited implantation on day 8, but injection on day 3 or 5 did not. Delayed implantation by A-LHRHγG injected on day 4 was overcome by concomitant administration of 2 subcutaneous injections of Iug of synthetic LHRH, or single subcutaneous injection of Iug of E2. The results indicate that the hypothalamic LHRH is essential on day 4 of pregnancy for timely implantation of fertilized ova, presumably by maintaining LH and consequently estrogen secretion from ovaries.
AB - Antibody against synthetic LHRH was generated in ewes by repeated intradermal injection with Glu(I)-LHRH coupled with human serum albumin and then separated anti-LHRH gamma globulin (A-LHRHγG) by ammonium sulphate precipitation technique. Daily injection of A-LHRHγG from day 1 through day 7 (1ml,iv), caused delayed implantation for 7-8 days and only 2 in 5 rats could maintain normal pregnancy course until term. Control rats in which normal sheep gamma globulin (NSG) was injected under the same protocol, could undergo normal fetal development. Daily injection of A-LHRHγG from day 3 through day 5 delayed the nidation process for 4-6 days. Plasma estrogen in the A-LHRHγG treated rats was maintained at low levels, and raised significantly on day 4 and 5 in NSG treated control rats. A single injection of 1 ml of A-LHRHγG on day 4 inhibited implantation on day 8, but injection on day 3 or 5 did not. Delayed implantation by A-LHRHγG injected on day 4 was overcome by concomitant administration of 2 subcutaneous injections of Iug of synthetic LHRH, or single subcutaneous injection of Iug of E2. The results indicate that the hypothalamic LHRH is essential on day 4 of pregnancy for timely implantation of fertilized ova, presumably by maintaining LH and consequently estrogen secretion from ovaries.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017726698&partnerID=8YFLogxK
M3 - Article
VL - 29
SP - 1717
EP - 1723
JO - Nippon Sanka Fujinka Gakkai zasshi
JF - Nippon Sanka Fujinka Gakkai zasshi
SN - 0300-9165
IS - 12
ER -