Inhibition of IL-2 production by Nil-2-a in murine T cells

Sabina Pucci, Gino Doria, Simona Barile, Claudio Pioli, Daniela Frasca

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The loss of IL-2 production is the main defect accounting for age-related immunodeficiencies. We have investigated the molecular mechanisms involved in the decrease of IL-2 production in CD4+ T cells from aging mice. Our results demonstrate that the stability of IL-2 mRNA increases in T cells from young mice, whereas it declines in T cells from old mice with the time of stimulation, suggesting the existence of different mechanisms of post-transcriptional regulation in young and old mice. We found that the IL-2 mRNA level in T cells from young but not from old mice increased up to 6- to 10-fold by addition of cycloheximide (CHX) while the stability of IL-2 mRNA is not affected. We then looked for IL-2 inducible inhibitory factors in T cells from young and old mice and demonstrated the presence of Nil-2-a, a zinc finger protein which negatively controls IL-2 gene transcription in human cells. This protein could be detected in T cells from both young and old mice, yet, in the presence of CHX, its binding activity was reduced by 75% in T cells from young but not from old mice. These findings show that Nil-2-a accounts for the negative control of IL-2 production in the mouse and explain the reduced IL-2 production in aging.

Original languageEnglish
Pages (from-to)1435-1440
Number of pages6
JournalInternational Immunology
Volume10
Issue number10
DOIs
StatePublished - Oct 14 1998
Externally publishedYes

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Interleukin-2
T-Lymphocytes
Cycloheximide
Messenger RNA
TCF Transcription Factors
Cell Aging
Zinc Fingers
Proteins
Genes

Keywords

  • Aging
  • IL-2 mRNA stability
  • Inhibitory nuclear factors
  • Nil-2-a

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of IL-2 production by Nil-2-a in murine T cells. / Pucci, Sabina; Doria, Gino; Barile, Simona; Pioli, Claudio; Frasca, Daniela.

In: International Immunology, Vol. 10, No. 10, 14.10.1998, p. 1435-1440.

Research output: Contribution to journalArticle

Pucci, Sabina ; Doria, Gino ; Barile, Simona ; Pioli, Claudio ; Frasca, Daniela. / Inhibition of IL-2 production by Nil-2-a in murine T cells. In: International Immunology. 1998 ; Vol. 10, No. 10. pp. 1435-1440.
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