Abstract
The loss of IL-2 production is the main defect accounting for age-related immunodeficiencies. We have investigated the molecular mechanisms involved in the decrease of IL-2 production in CD4+ T cells from aging mice. Our results demonstrate that the stability of IL-2 mRNA increases in T cells from young mice, whereas it declines in T cells from old mice with the time of stimulation, suggesting the existence of different mechanisms of post-transcriptional regulation in young and old mice. We found that the IL-2 mRNA level in T cells from young but not from old mice increased up to 6- to 10-fold by addition of cycloheximide (CHX) while the stability of IL-2 mRNA is not affected. We then looked for IL-2 inducible inhibitory factors in T cells from young and old mice and demonstrated the presence of Nil-2-a, a zinc finger protein which negatively controls IL-2 gene transcription in human cells. This protein could be detected in T cells from both young and old mice, yet, in the presence of CHX, its binding activity was reduced by 75% in T cells from young but not from old mice. These findings show that Nil-2-a accounts for the negative control of IL-2 production in the mouse and explain the reduced IL-2 production in aging.
| Original language | English |
|---|---|
| Pages (from-to) | 1435-1440 |
| Number of pages | 6 |
| Journal | International Immunology |
| Volume | 10 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 14 1998 |
| Externally published | Yes |
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Keywords
- Aging
- IL-2 mRNA stability
- Inhibitory nuclear factors
- Nil-2-a
ASJC Scopus subject areas
- Immunology
Cite this
Inhibition of IL-2 production by Nil-2-a in murine T cells. / Pucci, Sabina; Doria, Gino; Barile, Simona; Pioli, Claudio; Frasca, Daniela.
In: International Immunology, Vol. 10, No. 10, 14.10.1998, p. 1435-1440.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of IL-2 production by Nil-2-a in murine T cells
AU - Pucci, Sabina
AU - Doria, Gino
AU - Barile, Simona
AU - Pioli, Claudio
AU - Frasca, Daniela
PY - 1998/10/14
Y1 - 1998/10/14
N2 - The loss of IL-2 production is the main defect accounting for age-related immunodeficiencies. We have investigated the molecular mechanisms involved in the decrease of IL-2 production in CD4+ T cells from aging mice. Our results demonstrate that the stability of IL-2 mRNA increases in T cells from young mice, whereas it declines in T cells from old mice with the time of stimulation, suggesting the existence of different mechanisms of post-transcriptional regulation in young and old mice. We found that the IL-2 mRNA level in T cells from young but not from old mice increased up to 6- to 10-fold by addition of cycloheximide (CHX) while the stability of IL-2 mRNA is not affected. We then looked for IL-2 inducible inhibitory factors in T cells from young and old mice and demonstrated the presence of Nil-2-a, a zinc finger protein which negatively controls IL-2 gene transcription in human cells. This protein could be detected in T cells from both young and old mice, yet, in the presence of CHX, its binding activity was reduced by 75% in T cells from young but not from old mice. These findings show that Nil-2-a accounts for the negative control of IL-2 production in the mouse and explain the reduced IL-2 production in aging.
AB - The loss of IL-2 production is the main defect accounting for age-related immunodeficiencies. We have investigated the molecular mechanisms involved in the decrease of IL-2 production in CD4+ T cells from aging mice. Our results demonstrate that the stability of IL-2 mRNA increases in T cells from young mice, whereas it declines in T cells from old mice with the time of stimulation, suggesting the existence of different mechanisms of post-transcriptional regulation in young and old mice. We found that the IL-2 mRNA level in T cells from young but not from old mice increased up to 6- to 10-fold by addition of cycloheximide (CHX) while the stability of IL-2 mRNA is not affected. We then looked for IL-2 inducible inhibitory factors in T cells from young and old mice and demonstrated the presence of Nil-2-a, a zinc finger protein which negatively controls IL-2 gene transcription in human cells. This protein could be detected in T cells from both young and old mice, yet, in the presence of CHX, its binding activity was reduced by 75% in T cells from young but not from old mice. These findings show that Nil-2-a accounts for the negative control of IL-2 production in the mouse and explain the reduced IL-2 production in aging.
KW - Aging
KW - IL-2 mRNA stability
KW - Inhibitory nuclear factors
KW - Nil-2-a
UR - http://www.scopus.com/inward/record.url?scp=0031660614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031660614&partnerID=8YFLogxK
U2 - 10.1093/intimm/10.10.1435
DO - 10.1093/intimm/10.10.1435
M3 - Article
VL - 10
SP - 1435
EP - 1440
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 10
ER -