Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160

Tetsu Yano, Sinisa Radulovic, Yutaka Osuga, Koji Kugu, Hiroyuki Yoshikawa, Yuji Taketani, Andrew V Schally

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, we investigated the effects of somatostatin analog RC-160 on the growth of the OV-1063 human epithelial ovarian cancer cell line in vitro. RC-160 inhibited cell proliferation, as measured by cell number, and [3H]thymidine incorporation into DNA at 10-9-10-5 M. In OV-1063 cells, 125I-labeled RC-160 was bound to one class of specific, saturable binding sites with high affinity (K(d) = 0.2 ± 0.03 nM) and low capacity (5,500 binding sites per cell).125I-labeled RC-160 could be displaced by unlabeled RC-160. Ligand binding was dependent on time and temperature. Receptor internalization assay showed that the ligand-receptor complex was internalized at 37°, which indicates the presence of biologically active somatostatin receptors on OV-1063 cells. These results suggest that somatostatin analog RC-160 can suppress the growth of OV-1063 human epithelial ovarian cancer cells by a direct action and that the inhibitory effect of somatostatin analog is mediated through the high-affinity somatostatin receptors. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalOncology
Volume59
Issue numberSUPPL. 1
StatePublished - Dec 12 2000
Externally publishedYes

Fingerprint

Somatostatin
Growth
Somatostatin Receptors
Binding Sites
Ligands
Thymidine
vapreotide
Ovarian epithelial cancer
In Vitro Techniques
Cell Count
Cell Proliferation
Cell Line
Temperature
DNA

Keywords

  • Ovarian cancer
  • Somatostatin
  • Somatostatin analog
  • Somatostatin receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yano, T., Radulovic, S., Osuga, Y., Kugu, K., Yoshikawa, H., Taketani, Y., & Schally, A. V. (2000). Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160. Oncology, 59(SUPPL. 1), 45-49.

Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160. / Yano, Tetsu; Radulovic, Sinisa; Osuga, Yutaka; Kugu, Koji; Yoshikawa, Hiroyuki; Taketani, Yuji; Schally, Andrew V.

In: Oncology, Vol. 59, No. SUPPL. 1, 12.12.2000, p. 45-49.

Research output: Contribution to journalArticle

Yano, T, Radulovic, S, Osuga, Y, Kugu, K, Yoshikawa, H, Taketani, Y & Schally, AV 2000, 'Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160', Oncology, vol. 59, no. SUPPL. 1, pp. 45-49.
Yano T, Radulovic S, Osuga Y, Kugu K, Yoshikawa H, Taketani Y et al. Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160. Oncology. 2000 Dec 12;59(SUPPL. 1):45-49.
Yano, Tetsu ; Radulovic, Sinisa ; Osuga, Yutaka ; Kugu, Koji ; Yoshikawa, Hiroyuki ; Taketani, Yuji ; Schally, Andrew V. / Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160. In: Oncology. 2000 ; Vol. 59, No. SUPPL. 1. pp. 45-49.
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