Inhibition of HIV type 1 infection with a RANTES-IgG3 fusion protein

P. M. Challita-Eid, E. Klimatcheva, B. T. Day, T. Evans, K. Dreyer, B. J. Rimel, J. D. Rosenblatt, V. Planelles

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The natural ligands for the chemokine receptors CCR5 (RANTES, MIP-1α, and MIP-1β) and CXCR4 (SDF1) can act as potent inhibitors of infection by the human immunodeficiency virus type 1 (HIV-1) at the level of viral entry. Unlike antibody-mediated inhibition, chemokine-mediated inhibition is broadly effective. Different HIV-1 strains can utilize the same coreceptor(s) for viral entry and, therefore, can be blocked by the same chemokine(s). HIV-1 strains that are highly resistant to neutralization by V3-specific antibodies are sensitive to inhibition by chemokines. Therefore, the use of chemokine- derived molecules constitutes a potential therapeutic approach to prevent infection by HIV-1. We have generated a fusion protein between RANTES and human IgG3 (RANTES-IgG3). The effectiveness of RANTES-IgG3 inhibition of infection by HIV-1 was similar to that of rRANTES. Inhibition of HIV-1 by RANTES-IgG3 was specific for CCR5-dependent but not CXCR4-dependent HIV-1 isolates. Fusion of a chemokine to an IgG moiety offers two desirable properties with respect to the recombinant chemokine alone. First, IgG fusion proteins have extended half-lives in vivo. Second, molecules with IgG heavy chain moieties may be able to cross the placenta and potentially induce fetal protection.

Original languageEnglish (US)
Pages (from-to)1617-1624
Number of pages8
JournalAIDS research and human retroviruses
Volume14
Issue number18
DOIs
StatePublished - Jan 1 1998

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Challita-Eid, P. M., Klimatcheva, E., Day, B. T., Evans, T., Dreyer, K., Rimel, B. J., Rosenblatt, J. D., & Planelles, V. (1998). Inhibition of HIV type 1 infection with a RANTES-IgG3 fusion protein. AIDS research and human retroviruses, 14(18), 1617-1624. https://doi.org/10.1089/aid.1998.14.1617