Inhibition of growth of human small cell and non-small cell lung carcinomas by antagonists of growth hormone-releasing hormone (GH-RH)

Jacek Pinski, Andrew V Schally, Andreas Jungwirth, Kate Groot, Gabor Halmos, Patricia Armatis, Marta Zarandi, Manuel Vadillo-Buenfil

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Insulin-like growth factors-I and-II (IGF-I and IGF-II) may be involved in the proliferation of human lung carcinomas. The purpose of this study was to investigate the effects of two potent antagonists of growth hormone-releasing hormone (GH-RH), MZ-4-71 and MZ-5-156 on the growth of the H69 human small cell lung cancer (SCLC) and H157 non-SCLC (NSCLC) lines transplanted into nude mice or cultured in vitro. Nude mice bearing H69 and H157 tumors were treated for 3-5 weeks with MZ-4-71 or MZ-5-156 injected s.c. twice a day at a dose of 20 μg/animal. Growth of H69 and H157 tumors in nude mice was significantly inhibited by MZ-4-71 and MZ-5-156 as shown by a reduction in tumor volume and weight. In animals bearing H157 NSCLC, treatment with MZ-4-71 decreased IGF-I and IGF-II levels in tumor tissue. Levels of IGF-I, but not of IGF-II in serum and liver tissue of H157 tumor-bearing nude mice treated with MZ-4-71 were decreased. High affinity binding sites for IGF-I were demonstrated on membranes of H69 and H157 tumors. In cell cultures, the proliferation rate of H69 SCLC cells was suppressed by 10-7-10-5 M MZ-4-71, but H157 NSCLC line was only inhibited by 10-5 M antagonist. Our findings demonstrate that the GH-RH antagonists MZ-4-71 and MZ-5-156 can inhibit the growth of SCLC and NSCLC. This new approach to the management of lung cancer merits further investigation.

Original languageEnglish
Pages (from-to)1099-1105
Number of pages7
JournalInternational Journal of Oncology
Volume9
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Growth Hormone-Releasing Hormone
Non-Small Cell Lung Carcinoma
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Nude Mice
Growth
Small Cell Lung Carcinoma
Neoplasms
Tumor Burden
Hormone Antagonists
MZ 4-71
Lung Neoplasms
Cell Culture Techniques
Binding Sites
Cell Proliferation
Carcinoma
Lung
Membranes
MZ 5-156
Liver

Keywords

  • GH-RH antagonists
  • IGF-I
  • IGF-II
  • lung cancer
  • non-SCLC
  • SCLC

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibition of growth of human small cell and non-small cell lung carcinomas by antagonists of growth hormone-releasing hormone (GH-RH). / Pinski, Jacek; Schally, Andrew V; Jungwirth, Andreas; Groot, Kate; Halmos, Gabor; Armatis, Patricia; Zarandi, Marta; Vadillo-Buenfil, Manuel.

In: International Journal of Oncology, Vol. 9, No. 6, 01.12.1996, p. 1099-1105.

Research output: Contribution to journalArticle

Pinski, J, Schally, AV, Jungwirth, A, Groot, K, Halmos, G, Armatis, P, Zarandi, M & Vadillo-Buenfil, M 1996, 'Inhibition of growth of human small cell and non-small cell lung carcinomas by antagonists of growth hormone-releasing hormone (GH-RH)', International Journal of Oncology, vol. 9, no. 6, pp. 1099-1105.
Pinski, Jacek ; Schally, Andrew V ; Jungwirth, Andreas ; Groot, Kate ; Halmos, Gabor ; Armatis, Patricia ; Zarandi, Marta ; Vadillo-Buenfil, Manuel. / Inhibition of growth of human small cell and non-small cell lung carcinomas by antagonists of growth hormone-releasing hormone (GH-RH). In: International Journal of Oncology. 1996 ; Vol. 9, No. 6. pp. 1099-1105.
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