Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone

Alain Belanger, Fernand Labrie, Pierre Borgeat, Muriel Savary, Jean Cote, Jacques Drouin, Andrew V Schally, David H. Coy, Esther J. Coy, Hans Immer, K. Sestanj, V. Nelson, Manfred Gotz

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Addition of increasing doses of synthetic growth hormone-release inhibiting hormone (GH-RIH) leads to a progressive decrease of the basal and N6monobutyryl cyclic AMP-,theophylline- and prostaglandin E2-induced release of immunoreactive growth hormone (GH) and thyrotropin (TSH) release from rat anterior pituitary cells in monolayer culture. A halfmaximal effect is measured at 3 × 10-9 M GH-RIH while a maximal inhibition to 10-20% of the control level is found at 1 × 10-7 M. Using rat hemipituitaries and measurement of GH release by both polyacrylamide gel electrophoresis and radioimmunoassay, a maximal effect of GH-RIH was found in the first 5 min of incubation. The inhibitory effect of GH-RIH on GH release remained constant for at least 3 h. GH-RIH does not affect the basal or induced release of prolactin and luteinizing hormone nor the high K +-induced release of GH and TSH.

Original languageEnglish
Pages (from-to)329-339
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume1
Issue number5
DOIs
StatePublished - Jan 1 1974
Externally publishedYes

Fingerprint

Thyrotropin
Growth Hormone
Hormones
Rats
Level control
Theophylline
Luteinizing Hormone
Electrophoresis
Dinoprostone
Prolactin
Cyclic AMP
Radioimmunoassay
Polyacrylamide Gel Electrophoresis
Monolayers

Keywords

  • anterior pituitary
  • hypothalamic hormone
  • somatostatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone. / Belanger, Alain; Labrie, Fernand; Borgeat, Pierre; Savary, Muriel; Cote, Jean; Drouin, Jacques; Schally, Andrew V; Coy, David H.; Coy, Esther J.; Immer, Hans; Sestanj, K.; Nelson, V.; Gotz, Manfred.

In: Molecular and Cellular Endocrinology, Vol. 1, No. 5, 01.01.1974, p. 329-339.

Research output: Contribution to journalArticle

Belanger, A, Labrie, F, Borgeat, P, Savary, M, Cote, J, Drouin, J, Schally, AV, Coy, DH, Coy, EJ, Immer, H, Sestanj, K, Nelson, V & Gotz, M 1974, 'Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone', Molecular and Cellular Endocrinology, vol. 1, no. 5, pp. 329-339. https://doi.org/10.1016/0303-7207(74)90022-7
Belanger, Alain ; Labrie, Fernand ; Borgeat, Pierre ; Savary, Muriel ; Cote, Jean ; Drouin, Jacques ; Schally, Andrew V ; Coy, David H. ; Coy, Esther J. ; Immer, Hans ; Sestanj, K. ; Nelson, V. ; Gotz, Manfred. / Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone. In: Molecular and Cellular Endocrinology. 1974 ; Vol. 1, No. 5. pp. 329-339.
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