Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone

Alain Belanger, Fernand Labrie, Pierre Borgeat, Muriel Savary, Jean Cote, Jacques Drouin, Andrew V. Schally, David H. Coy, Esther J. Coy, Hans Immer, K. Sestanj, V. Nelson, Manfred Gotz

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Abstract

Addition of increasing doses of synthetic growth hormone-release inhibiting hormone (GH-RIH) leads to a progressive decrease of the basal and N6monobutyryl cyclic AMP-,theophylline- and prostaglandin E2-induced release of immunoreactive growth hormone (GH) and thyrotropin (TSH) release from rat anterior pituitary cells in monolayer culture. A halfmaximal effect is measured at 3 × 10-9 M GH-RIH while a maximal inhibition to 10-20% of the control level is found at 1 × 10-7 M. Using rat hemipituitaries and measurement of GH release by both polyacrylamide gel electrophoresis and radioimmunoassay, a maximal effect of GH-RIH was found in the first 5 min of incubation. The inhibitory effect of GH-RIH on GH release remained constant for at least 3 h. GH-RIH does not affect the basal or induced release of prolactin and luteinizing hormone nor the high K +-induced release of GH and TSH.

Original languageEnglish (US)
Pages (from-to)329-339
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume1
Issue number5
DOIs
StatePublished - Oct 1974
Externally publishedYes

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Keywords

  • anterior pituitary
  • hypothalamic hormone
  • somatostatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Belanger, A., Labrie, F., Borgeat, P., Savary, M., Cote, J., Drouin, J., Schally, A. V., Coy, D. H., Coy, E. J., Immer, H., Sestanj, K., Nelson, V., & Gotz, M. (1974). Inhibition of growth hormone and thyrotropin release by growth hormone-release inhibiting hormone. Molecular and Cellular Endocrinology, 1(5), 329-339. https://doi.org/10.1016/0303-7207(74)90022-7