Inhibition of growth and guanylate cyclase activity of an undifferentiated prostate adenocarcinoma by an extract of the balsam pear (Momordica charantia abbreviata)

A. J. Claflin, D. L. Vesely, J. L. Hudson, C. B. Bagwell, D. C. Lehotay, T. M. Lo, M. A. Fletcher, Norman L Block, G. S. Levey

Research output: Contribution to journalArticle

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Abstract

We have recently described the presence of a guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] inhibitor (GCI) in an aqueous extract of the balsam pear (Momordica charantia abbreviata). Because the guanylate cyclase-cyclic GMP system is though to be involved in cell growth, DNA and RNA synthesis, and possibly malignant transformation, we examined the effect of the aqueous extract containing GCI on an undifferentiated adenocarcinoma of the rat prostate and concanavalin-A-stimulated [ 3H]thymidine incorporation into cultured splenic lymphocytes, a process thought to be mediated by cyclic GMP. The results demonstrate that the extract of the balsam pear blocks both the growth of the rat prostatic adenocarcinoma in vitro and [ 3H]thymidine incorporation into DNA. DNA histograms from flow cytometry indicated that the extract containing GCI inhibited in the G 2+ M phase of the cell cycle, a presumed locus of cyclic GMP effects. In addition, guanylate cyclase activity was significantly greater in the tumor than normal prostate tissue and was decreased by the extract containing GCI. Cyclic GMP levels in the tumor in culture were also decreased by addition of the extract. It remains to be determined whether or not the anti-tumor agent and GCI are the same substance.

Original languageEnglish
Pages (from-to)989-993
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number2
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint

Balsams
Momordica charantia
Pyrus
Guanylate Cyclase
Cyclic GMP
Prostate
Adenocarcinoma
Growth
Thymidine
DNA
Neoplasms
Concanavalin A
Cell Division
Cell Cycle
Flow Cytometry
Lymphocytes
RNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Inhibition of growth and guanylate cyclase activity of an undifferentiated prostate adenocarcinoma by an extract of the balsam pear (Momordica charantia abbreviata). / Claflin, A. J.; Vesely, D. L.; Hudson, J. L.; Bagwell, C. B.; Lehotay, D. C.; Lo, T. M.; Fletcher, M. A.; Block, Norman L; Levey, G. S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, No. 2, 01.12.1978, p. 989-993.

Research output: Contribution to journalArticle

Claflin, A. J. ; Vesely, D. L. ; Hudson, J. L. ; Bagwell, C. B. ; Lehotay, D. C. ; Lo, T. M. ; Fletcher, M. A. ; Block, Norman L ; Levey, G. S. / Inhibition of growth and guanylate cyclase activity of an undifferentiated prostate adenocarcinoma by an extract of the balsam pear (Momordica charantia abbreviata). In: Proceedings of the National Academy of Sciences of the United States of America. 1978 ; Vol. 75, No. 2. pp. 989-993.
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