Inhibition of dopamine and choline acetyltransferase concentrations in Rat CNS neurons by rat α1- and α2-macroglobulins

Yi Qun Hu, Daniel J. Liebl, Dean E. Dluzen, Peter H. Koo

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Previous studies have implicated human alpha-2-macroglobulin (α2M) as a potential regulator of neuronal development and function. Rat alpha-1- macroglobulin (α1M) and acute-phase alpha-2-macroglobulin (α2M) are murine homologues of human α2M. In this report, we tested the effect of intracranially infused serotonin-activated rat α1M (5HT-α1M) on the concentration of dopamine (DA) in the corpus striatum in vivo and the effect of 5HT-activated rat α1M and α2M on the choline acetyltransferase (CHAT) activity upon embryonic basal forebrain neurons in culture. The results show that direct infusion of 0.65 nmole rat 5HT-α1M into the adult rat corpus striatum produced a consistent attenuation upon striatal DA concentrations. This decrease was particularly prominent at 5-7 days post-infusion. In addition, rat 5HT-α1M and rat 5HT-α2M, like human 5HT-α2M, all significantly inhibited ChAT activity of embryonic rat cerebral cortex neurons. Although normal human α2M and rat α2M were either marginally or insignificantly inhibitory in this preparation, normal rat α1M dose- dependently inhibited ChAT activity. These results demonstrate that monoamine-activated α-macroglobulins from rat depress dopaminergic and cholinergic neurotransmitter systems in the CNS, and this suggests a potential regulatory role of these alphamacrogiobulins in neurotransmitter metabolism.

Original languageEnglish (US)
Pages (from-to)541-550
Number of pages10
JournalJournal of Neuroscience Research
Volume51
Issue number4
DOIs
StatePublished - Feb 15 1998

Keywords

  • Acetylcholine
  • Alzheimer's disease
  • Monoamines
  • Neurotransmitter
  • Neurotrophin
  • Nigrostriatum
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

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