Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate

Herman S Cheung, John D. Sallis, Peter G. Mitchell, Janine A. Struve

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number1
DOIs
StatePublished - Aug 31 1990
Externally publishedYes

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Crystals
Somatomedins
Transcription
Durapatite
Crystallization
S Phase
Thymidine
Cell Cycle
Fibroblasts
DNA
Serum
Cells
Plasmas
phosphocitrate
calcium phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate. / Cheung, Herman S; Sallis, John D.; Mitchell, Peter G.; Struve, Janine A.

In: Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 31.08.1990, p. 20-25.

Research output: Contribution to journalArticle

Cheung, Herman S ; Sallis, John D. ; Mitchell, Peter G. ; Struve, Janine A. / Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 171, No. 1. pp. 20-25.
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