Abstract
Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 20-25 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 171 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 31 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate. / Cheung, Herman S; Sallis, John D.; Mitchell, Peter G.; Struve, Janine A.
In: Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 31.08.1990, p. 20-25.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate
AU - Cheung, Herman S
AU - Sallis, John D.
AU - Mitchell, Peter G.
AU - Struve, Janine A.
PY - 1990/8/31
Y1 - 1990/8/31
N2 - Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.
AB - Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.
UR - http://www.scopus.com/inward/record.url?scp=0025091355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025091355&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91350-2
DO - 10.1016/0006-291X(90)91350-2
M3 - Article
C2 - 2118343
AN - SCOPUS:0025091355
VL - 171
SP - 20
EP - 25
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -