Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate

Herman S. Cheung; John D. Sallis; Peter G. Mitchell; Janine A. Struve

doi:10.1016/0006-291X(90)91350-2

Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate

Herman S. Cheung, John D. Sallis, Peter G. Mitchell, Janine A. Struve

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.

Original language	English (US)
Pages (from-to)	20-25
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	171
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91350-2
State	Published - Aug 31 1990
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.",

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note = "Funding Information: in part by USPHS grants Center Grant. We gratefully McCarty and L.M. Ryan. Funding Information: This work was supported Arthritis Foundation Research helpful comments of Drs. D.J.",

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AU - Cheung, Herman S.

AU - Sallis, John D.

AU - Mitchell, Peter G.

AU - Struve, Janine A.

N1 - Funding Information: in part by USPHS grants Center Grant. We gratefully McCarty and L.M. Ryan. Funding Information: This work was supported Arthritis Foundation Research helpful comments of Drs. D.J.

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N2 - Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.

AB - Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.

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Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate

Abstract

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