Abstract
Basic calcium phosphate crystals control the traverse of cells from the Go G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.
Original language | English (US) |
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Pages (from-to) | 20-25 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 171 |
Issue number | 1 |
DOIs | |
State | Published - Aug 31 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology