Inhibition by rhodamine 123 of protein synthesis in mitochondria of normal and cancer tissues

Wafa H. Abou-Khalil, Grace K. Arimura, Adel A. Yunis, Samir Abou-Khalil

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The effect of the mitochondrial dye rhodamine 123 (Rho 123) on protein synthesis (PS) activity was investigated in mitochondria isolated from liver and from both chloroma and erythroleukemia tumors. Incorporation of labelled leucine into mitochondrial protein was used to measure the rate of PS. While PS specific activity was much higher in hematopoietic tumors mitochondria as compared to that of liver, the addition of increased concentration of Rho 123 in all tested organelles resulted in increased inhibition of PS to reach 75-82% with 10 μg/ml of the dye. Similar results were obtained with 10 μg/ml of chloramphenicol, the specific inhibitor of mitochondrial PS. Moreover, under the conditions of the study, the addition of Rho 123 to mitochondria did not trigger any ATPase activity, thus eliminating any competition for the energy source ATP between PS and ATPase. These results demonstrate that, in addition to its known inhibitory action on oxidative phosphorylation, the mitochondrial dye Rho 123 has a potent inhibitory effect on PS in both liver and hematopoietic tumors mitochondria.

Original languageEnglish (US)
Pages (from-to)759-765
Number of pages7
JournalBiochemical and biophysical research communications
Issue number2
StatePublished - Jun 13 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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