Infrared photostimulation of the crista ampullaris

Suhrud M Rajguru, Claus Peter Richter, Agnella I. Matic, Gay R. Holstein, Stephen M. Highstein, Gregory M. Dittami, Richard D. Rabbitt

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The present results show that the semicircular canal crista ampullaris of the toadfish, Opsanus tau, is sensitive to infrared radiation (IR) applied in vivo. IR pulse trains (∼1862 nm, ∼200 μs pulse -1) delivered to the sensory epithelium by an optical fibre evoked profound changes in phasic and tonic discharge rates of postsynaptic afferent neurons. Phasic afferent responses to pulsed IR occurred with a latency of <8 ms while tonic responses developed with a time constant (τ) of 7 ms to 10 s following the onset or cessation of the radiation. Afferents responded to direct optical radiation of the sensory epithelium but did not respond to thermal stimuli that generated nearly equivalent temperature increases of the whole organ. A subset of afferent neurons fired an action potential in response to each IR pulse delivered to the sensory epithelium, at phase-locked rates up to 96 pulses per second. The latency between IR pulses and afferent nerve action potentials was much greater than synaptic delay and spike generation, demonstrating the presence of a signalling delay interposed between the IR pulse and the action potential. The same IR stimulus applied to afferent nerve axons failed to evoke responses of similar magnitude and failed to phase-lock afferent nerve action potentials. The present data support the hypothesis that pulsed IR activates sensory hair cells, thus leading to modulation of synaptic transmission and afferent nerve discharge reported here.

Original languageEnglish
Pages (from-to)1283-1294
Number of pages12
JournalJournal of Physiology
Volume589
Issue number6
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

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Semicircular Ducts
Radiation
Action Potentials
Batrachoidiformes
Afferent Neurons
Epithelium
Optical Fibers
Semicircular Canals
Synaptic Transmission
Axons

ASJC Scopus subject areas

  • Physiology

Cite this

Rajguru, S. M., Richter, C. P., Matic, A. I., Holstein, G. R., Highstein, S. M., Dittami, G. M., & Rabbitt, R. D. (2011). Infrared photostimulation of the crista ampullaris. Journal of Physiology, 589(6), 1283-1294. https://doi.org/10.1113/jphysiol.2010.198333

Infrared photostimulation of the crista ampullaris. / Rajguru, Suhrud M; Richter, Claus Peter; Matic, Agnella I.; Holstein, Gay R.; Highstein, Stephen M.; Dittami, Gregory M.; Rabbitt, Richard D.

In: Journal of Physiology, Vol. 589, No. 6, 01.03.2011, p. 1283-1294.

Research output: Contribution to journalArticle

Rajguru, SM, Richter, CP, Matic, AI, Holstein, GR, Highstein, SM, Dittami, GM & Rabbitt, RD 2011, 'Infrared photostimulation of the crista ampullaris', Journal of Physiology, vol. 589, no. 6, pp. 1283-1294. https://doi.org/10.1113/jphysiol.2010.198333
Rajguru SM, Richter CP, Matic AI, Holstein GR, Highstein SM, Dittami GM et al. Infrared photostimulation of the crista ampullaris. Journal of Physiology. 2011 Mar 1;589(6):1283-1294. https://doi.org/10.1113/jphysiol.2010.198333
Rajguru, Suhrud M ; Richter, Claus Peter ; Matic, Agnella I. ; Holstein, Gay R. ; Highstein, Stephen M. ; Dittami, Gregory M. ; Rabbitt, Richard D. / Infrared photostimulation of the crista ampullaris. In: Journal of Physiology. 2011 ; Vol. 589, No. 6. pp. 1283-1294.
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