Infrared microscopic imaging of cutaneous wound healing: Lipid conformation in the migrating epithelial tongue

Guo Yu, Olivera Stojadinovic, Marjana Tomic-Canic, Carol R. Flach, Richard Mendelsohn

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties revealed the presence of a lipid class with disordered chains within and in the vicinity of the migrating epithelial tongue. The presence of lipid ester C = O bands colocalized with the disordered chains provided evidence for the presence of carbonyl-containing lipid species. Gene array data complemented the biophysical studies and provided a biological rationale for the generation of the disordered chain species. This is the first clear observation, to our knowledge, of disordered lipid involvement in cutaneous wound healing. Several possibilities are discussed for the biological relevance of these observations.

Original languageEnglish (US)
Article number096009
JournalJournal of Biomedical Optics
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2012

Keywords

  • infrared imaging
  • lipid conformational order
  • migrating epithelial tongue
  • wound healing

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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