Influence of poly(propylene sulfide-block-ethylene glycol) di- And triblock copolymer architecture on the formation of molecular adlayers on gold surfaces and their effect on protein resistance: A candidate for surface modification in biosensor research

Lydia M. Feller, Simona Cerritelli, Marcus Textor, Jeffrey A. Hubbell, Samuele G.P. Tosatti

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Control of nonspecific interactions between bioanalytical surfaces and proteins in the analyte is critical in the design of biosensor systems. Here we explore poly(propylene sulfide-block-ethylene glycol) (PPS-PEG) di- and triblock copolymer adlayers on gold to gain such control. Chemisorption of the PPS block permits a simple dip-and-rinse coating process. We synthesized different architectures of di- and triblock copolymers, varying the molecular weight of PEG between 1.1 and 5 kDa while keeping the PPS block constant at around 4 kDa, thus permitting systematic variations in ethylene glycol surface density in the adlayer. A simple dip-and-rinse process was used to produce PPS-PEG adlayers on gold substrates, which were characterized with surface plasmon resonance (SPR) and further confirmed by ex situ variable angle spectral ellipsometry (VASE), and X-ray photoelectron spectroscopy (XPS). Crowding in the PPS chemisorbed layer seemed to limit the polymer adsorption process. Subsequent exposure of PPS-PEG adlayers to protein adsorption (human serum albumin at 1 mg/mL or full-concentration human serum) was monitored with in situ SPR. Protein adsorption can be reduced up to 97% for human serum albumin and up to 96% for blood serum relative to bare gold substrates. Triblock copolymers were more effective than corresponding diblocks. The possibility to render gold surfaces bioinert is the basis for application in bioanalytical devices.

Original languageEnglish (US)
Pages (from-to)10503-10510
Number of pages8
JournalMacromolecules
Volume38
Issue number25
DOIs
StatePublished - Dec 13 2005

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Influence of poly(propylene sulfide-block-ethylene glycol) di- And triblock copolymer architecture on the formation of molecular adlayers on gold surfaces and their effect on protein resistance: A candidate for surface modification in biosensor research'. Together they form a unique fingerprint.

Cite this