TY - JOUR
T1 - Influence of laminin substratum on cell proliferation and CALC I gene expression in medullary thyroid carcinoma C cell lines
AU - Lekmine, F.
AU - Lausson, S.
AU - Pidoux, E.
AU - Segond, N.
AU - Roos, B.
AU - Treilhou-Lahille, F.
AU - Jeanne, N.
N1 - Funding Information:
The authors wish to thank Paula Harry for her kind help with the English language. This work was supported by a grant (no. 6424) given by the Association pour la Recherche sur le Cancer (ARC) to F.T.L.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/11/25
Y1 - 1999/11/25
N2 - Medullary thyroid carcinoma (MTC) originates from C cells, which secrete calcitonin (CT) and CT gene-related peptide (CGRP), the two splice peptide products of the CALC I gene. Normal and hyperplastic C cells are intrafollicular, in contact with the basement membrane (BM) that is maintained around the differentiated tumors. To investigate the relationships between MTC evolution and BM constituents, we examined the modifications induced by laminin-1 and -2 (merosin), two isoforms colocalized in the follicular BM, on three MTC cell lines: murine rMTC 6-23 and CA-77 cells, and human TT cells. Laminin exerted a mitogenic activity on rMTC 6-23 and on TT cells, causing a concurrent decrease in both CT and CGRP mRNA levels and production of the peptides. Conversely, laminin reduced the proliferation rate and enhanced CGRP synthesis and secretion in CA-77 cells. This antiproliferative response, which coincides with an increase in differentiation markers, is comparable to that reported in normal cells and also in the neoplastic Caco-2 cell line. This suggests that laminin could exert opposite effects depending on the stage of tumor evolution. Copyright (C) 1999 Elsevier Science Ireland Ltd.
AB - Medullary thyroid carcinoma (MTC) originates from C cells, which secrete calcitonin (CT) and CT gene-related peptide (CGRP), the two splice peptide products of the CALC I gene. Normal and hyperplastic C cells are intrafollicular, in contact with the basement membrane (BM) that is maintained around the differentiated tumors. To investigate the relationships between MTC evolution and BM constituents, we examined the modifications induced by laminin-1 and -2 (merosin), two isoforms colocalized in the follicular BM, on three MTC cell lines: murine rMTC 6-23 and CA-77 cells, and human TT cells. Laminin exerted a mitogenic activity on rMTC 6-23 and on TT cells, causing a concurrent decrease in both CT and CGRP mRNA levels and production of the peptides. Conversely, laminin reduced the proliferation rate and enhanced CGRP synthesis and secretion in CA-77 cells. This antiproliferative response, which coincides with an increase in differentiation markers, is comparable to that reported in normal cells and also in the neoplastic Caco-2 cell line. This suggests that laminin could exert opposite effects depending on the stage of tumor evolution. Copyright (C) 1999 Elsevier Science Ireland Ltd.
KW - Calcitonin
KW - Calcitonin gene-related peptide
KW - Extracellular matrix
KW - Laminin
KW - Medullary thyroid carcinoma
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U2 - 10.1016/S0303-7207(99)00138-0
DO - 10.1016/S0303-7207(99)00138-0
M3 - Article
C2 - 10619409
AN - SCOPUS:0032701609
VL - 157
SP - 181
EP - 189
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -
