Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells

R. Jakesz, C. A. Smith, S. Aitken, K. Huff, W. Schuette, S. Shackney, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

In the present study, the effects of cell cycle phase and proliferation rate on the expression of specific estrogen binding activity were explored in hormone-dependent human breast cancer cells. A technique was developed to alter the proliferative rate of MCF-7 cells by plating at different densities. The doubling time ranged from 20 to 48 hr, showing a negative relation to the number of plated cells. Slowly proliferating cells had accumulated more than twice as much estrogen receptor (ER) activity as did fast-proliferating cells. Exposure of exponentially growing cells to isoleucine-deficient medium resulted in decreased thymidine incorporation and disappearance of detectable cellular ER activity. Overall protein synthesis was reduced by only 30% in cells growing in isoleucine-free medium. At 24 hr after release from isoleucine deprivation, ER levels are fully restored, although thymidine incorporation does not resume for an additional 6 to 8 hr, and increases in cell number are not seen for 24 hr. Exposure of exponentially growing cells to 2 mM thymidine for 24 hr produced partially synchronized MCF-7 cells (~70%). Six hr after release from excess thymidine, cells reached S phase; after 9 hr, G2; and after 18 hr, G1. ER levels immediately and, 6 hr after release, remained unchanged, showed a slight increase at 9 hr, and showed an increase of about 50 to 60% at 18 hr. These data suggest that: (a) ER binding activity and DNA synthesis can be dissociated; (b) ongoing protein synthesis is necessary for maintenance of cellular ER activity; and (c) ER is apparently synthesized throughout the cell cycle, with some evidence that this is predominantly in G1 and G2.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalCancer Research
Volume44
Issue number2
StatePublished - Apr 5 1984
Externally publishedYes

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Estrogen Receptors
Cell Cycle
Cell Proliferation
Breast Neoplasms
Thymidine
Isoleucine
MCF-7 Cells
Cell Count
S Phase
Estrogens
Proteins
Maintenance
Hormones
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Jakesz, R., Smith, C. A., Aitken, S., Huff, K., Schuette, W., Shackney, S., & Lippman, M. E. (1984). Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells. Cancer Research, 44(2), 619-625.

Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells. / Jakesz, R.; Smith, C. A.; Aitken, S.; Huff, K.; Schuette, W.; Shackney, S.; Lippman, Marc E.

In: Cancer Research, Vol. 44, No. 2, 05.04.1984, p. 619-625.

Research output: Contribution to journalArticle

Jakesz, R, Smith, CA, Aitken, S, Huff, K, Schuette, W, Shackney, S & Lippman, ME 1984, 'Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells', Cancer Research, vol. 44, no. 2, pp. 619-625.
Jakesz R, Smith CA, Aitken S, Huff K, Schuette W, Shackney S et al. Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells. Cancer Research. 1984 Apr 5;44(2):619-625.
Jakesz, R. ; Smith, C. A. ; Aitken, S. ; Huff, K. ; Schuette, W. ; Shackney, S. ; Lippman, Marc E. / Influence of cell proliferation and cell cycle phase on expression of estrogen receptor in MCF-7 breast cancer cells. In: Cancer Research. 1984 ; Vol. 44, No. 2. pp. 619-625.
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