Influence of accessory cell and T cell surface antigens on mitogen-induced IL 2 receptor expression

T. R. Malek, C. Chan, L. H. Glimcher, R. N. Germain, E. M. Shevach

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We have assessed the inhibitory effects of various monoclonal antibodies on the expression of the IL 2 receptor. Anti-LFA-1, but not anti-Ly-2, markedly inhibited the induction of the IL 2 receptor on the Ly-2+ subset. T-depleted spleen cells, L cells, and B lymphoma cells all functional as potent accessory cells (AC) for the induction of the IL 2 receptor on L3T4+ T cells. Anti-LFA-1 inhibited the induction of the IL 2 receptor irrespective of the type of AC used. Anti-L3T4 only inhibited the induction of IL 2 receptor expression when L cells were the source of AC. The inhibitory capacity of anti-L3T4 was not related to the expression of Ia on the AC population, because the magnitude of inhibition was comparable in cultures containing either Ia+ or Ia- L cells, whereas no inhibition was seen with either Ia+ or Ia- B lymphoma cells. We conclude from these studies that LFA-1 plays a critical role in mitogen-induced activation of both T cell subsets by promoting both T-Ac and T-T interactions. Although anti-L3T4 can inhibit T cell activation in the absence of the recognition of Ia, the mechanisms of inhibition and the proposed target molecule for L3T4 on the AC or the T cell have not been determined in our studies. A number of different models for the function of this cell surface antigen are discussed.

Original languageEnglish (US)
Pages (from-to)1826-1833
Number of pages8
JournalJournal of Immunology
Volume135
Issue number3
StatePublished - Dec 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Malek, T. R., Chan, C., Glimcher, L. H., Germain, R. N., & Shevach, E. M. (1985). Influence of accessory cell and T cell surface antigens on mitogen-induced IL 2 receptor expression. Journal of Immunology, 135(3), 1826-1833.