Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice

Loredana G. Bucciarelli, Andreas Pollreisz, Moritz Kebschull, Anjali Ganda, Anastasia Z. Kalea, Barry Hudson, Shan Zou Yu, Evanthia Lalla, Ravichandran Ramasamy, Paolo C. Colombo, Ann Marie Schmidt, Fang Yan Shi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice. Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture. Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

Original languageEnglish
Pages (from-to)249-261
Number of pages13
JournalDiabetes and Vascular Disease Research
Volume6
Issue number4
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Fingerprint

Endothelial Cells
Messenger RNA
Gene Expression Profiling
Endothelium
Real-Time Polymerase Chain Reaction
Apoptosis
Wounds and Injuries
Genes

Keywords

  • Artery
  • Diabetes
  • Endothelium
  • Inflammation
  • Vein

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice. / Bucciarelli, Loredana G.; Pollreisz, Andreas; Kebschull, Moritz; Ganda, Anjali; Kalea, Anastasia Z.; Hudson, Barry; Yu, Shan Zou; Lalla, Evanthia; Ramasamy, Ravichandran; Colombo, Paolo C.; Schmidt, Ann Marie; Shi, Fang Yan.

In: Diabetes and Vascular Disease Research, Vol. 6, No. 4, 01.10.2009, p. 249-261.

Research output: Contribution to journalArticle

Bucciarelli, LG, Pollreisz, A, Kebschull, M, Ganda, A, Kalea, AZ, Hudson, B, Yu, SZ, Lalla, E, Ramasamy, R, Colombo, PC, Schmidt, AM & Shi, FY 2009, 'Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice', Diabetes and Vascular Disease Research, vol. 6, no. 4, pp. 249-261. https://doi.org/10.1177/1479164109338775
Bucciarelli, Loredana G. ; Pollreisz, Andreas ; Kebschull, Moritz ; Ganda, Anjali ; Kalea, Anastasia Z. ; Hudson, Barry ; Yu, Shan Zou ; Lalla, Evanthia ; Ramasamy, Ravichandran ; Colombo, Paolo C. ; Schmidt, Ann Marie ; Shi, Fang Yan. / Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice. In: Diabetes and Vascular Disease Research. 2009 ; Vol. 6, No. 4. pp. 249-261.
@article{6d02bcd5f2b1443a97248cffc53984ac,
title = "Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice",
abstract = "Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice. Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture. Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.",
keywords = "Artery, Diabetes, Endothelium, Inflammation, Vein",
author = "Bucciarelli, {Loredana G.} and Andreas Pollreisz and Moritz Kebschull and Anjali Ganda and Kalea, {Anastasia Z.} and Barry Hudson and Yu, {Shan Zou} and Evanthia Lalla and Ravichandran Ramasamy and Colombo, {Paolo C.} and Schmidt, {Ann Marie} and Shi, {Fang Yan}",
year = "2009",
month = "10",
day = "1",
doi = "10.1177/1479164109338775",
language = "English",
volume = "6",
pages = "249--261",
journal = "Diabetes and Vascular Disease Research",
issn = "1479-1641",
publisher = "SAGE Publications Ltd",
number = "4",

}

TY - JOUR

T1 - Inflammatory stress in primary venous and aortic endothelial cells of type I diabetic mice

AU - Bucciarelli, Loredana G.

AU - Pollreisz, Andreas

AU - Kebschull, Moritz

AU - Ganda, Anjali

AU - Kalea, Anastasia Z.

AU - Hudson, Barry

AU - Yu, Shan Zou

AU - Lalla, Evanthia

AU - Ramasamy, Ravichandran

AU - Colombo, Paolo C.

AU - Schmidt, Ann Marie

AU - Shi, Fang Yan

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice. Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture. Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

AB - Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice. Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture. Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-1 mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.

KW - Artery

KW - Diabetes

KW - Endothelium

KW - Inflammation

KW - Vein

UR - http://www.scopus.com/inward/record.url?scp=70350152746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350152746&partnerID=8YFLogxK

U2 - 10.1177/1479164109338775

DO - 10.1177/1479164109338775

M3 - Article

C2 - 20368219

AN - SCOPUS:70350152746

VL - 6

SP - 249

EP - 261

JO - Diabetes and Vascular Disease Research

JF - Diabetes and Vascular Disease Research

SN - 1479-1641

IS - 4

ER -