Inflammatory cytokines induce specific time- and concentration-dependent MicroRNA release by chondrocytes, synoviocytes, and meniscus cells

Amaris A. Genemaras, Hayley Ennis, Lee Kaplan, Chun-Yuh Huang

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19 Citations (Scopus)

Abstract

In knee osteoarthritis (OA), concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α increase in joint tissues and synovial fluid which incite a catabolic cascade and further the progression of OA. Several microRNAs (miRNA) have been associated with apoptosis (miR-16), inflammation (miR-22, miR-146a), and matrix degradation (miR-140, miR-27b) in developed OA or its symptoms. In this study, the time- and concentration-dependent nature of cellular and extracellular miRNAs in synoviocytes, meniscus cells, and chondrocytes as influenced by inflammatory cytokines was investigated. For time-dependent studies, three cell types were stimulated with 10ng/ml IL-1β or 50ng/ml TNF-α for 8, 16, and 24h. For concentration-dependent studies, chondrocytes were stimulated with a higher level of IL-1β (20ng/ml) or TNF-α (100ng/ml) for 8h. Cellular and extracellular expressions of miR-22, miR-16, miR-146a, miR-27b, and miR-140 were analyzed by RT-PCR. Time-dependent cellular miRNA expressions were similar across the three cell types with miR-146a significantly up-regulated and miR-27b significantly down-regulated at all time points. However, chondrocytes exhibited a unique extracellular miRNA profile with an increased release rate of miR-27b at 24h. Our findings support further research into the characterization of miRNAs in synovial fluid for the development of early detection strategies of OA or cartilage injury.

Original languageEnglish (US)
JournalJournal of Orthopaedic Research
DOIs
StateAccepted/In press - 2015

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Chondrocytes
MicroRNAs
Cytokines
Interleukin-1
Osteoarthritis
Tumor Necrosis Factor-alpha
Synovial Fluid
Knee Osteoarthritis
Cartilage
Joints
Meniscus
Synoviocytes
Apoptosis
Inflammation
Polymerase Chain Reaction
Wounds and Injuries
Research

Keywords

  • Chondrocytes
  • Inflammatory cytokines
  • MicroRNA
  • Osteoarthritis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

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title = "Inflammatory cytokines induce specific time- and concentration-dependent MicroRNA release by chondrocytes, synoviocytes, and meniscus cells",
abstract = "In knee osteoarthritis (OA), concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α increase in joint tissues and synovial fluid which incite a catabolic cascade and further the progression of OA. Several microRNAs (miRNA) have been associated with apoptosis (miR-16), inflammation (miR-22, miR-146a), and matrix degradation (miR-140, miR-27b) in developed OA or its symptoms. In this study, the time- and concentration-dependent nature of cellular and extracellular miRNAs in synoviocytes, meniscus cells, and chondrocytes as influenced by inflammatory cytokines was investigated. For time-dependent studies, three cell types were stimulated with 10ng/ml IL-1β or 50ng/ml TNF-α for 8, 16, and 24h. For concentration-dependent studies, chondrocytes were stimulated with a higher level of IL-1β (20ng/ml) or TNF-α (100ng/ml) for 8h. Cellular and extracellular expressions of miR-22, miR-16, miR-146a, miR-27b, and miR-140 were analyzed by RT-PCR. Time-dependent cellular miRNA expressions were similar across the three cell types with miR-146a significantly up-regulated and miR-27b significantly down-regulated at all time points. However, chondrocytes exhibited a unique extracellular miRNA profile with an increased release rate of miR-27b at 24h. Our findings support further research into the characterization of miRNAs in synovial fluid for the development of early detection strategies of OA or cartilage injury.",
keywords = "Chondrocytes, Inflammatory cytokines, MicroRNA, Osteoarthritis",
author = "Genemaras, {Amaris A.} and Hayley Ennis and Lee Kaplan and Chun-Yuh Huang",
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T1 - Inflammatory cytokines induce specific time- and concentration-dependent MicroRNA release by chondrocytes, synoviocytes, and meniscus cells

AU - Genemaras, Amaris A.

AU - Ennis, Hayley

AU - Kaplan, Lee

AU - Huang, Chun-Yuh

PY - 2015

Y1 - 2015

N2 - In knee osteoarthritis (OA), concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α increase in joint tissues and synovial fluid which incite a catabolic cascade and further the progression of OA. Several microRNAs (miRNA) have been associated with apoptosis (miR-16), inflammation (miR-22, miR-146a), and matrix degradation (miR-140, miR-27b) in developed OA or its symptoms. In this study, the time- and concentration-dependent nature of cellular and extracellular miRNAs in synoviocytes, meniscus cells, and chondrocytes as influenced by inflammatory cytokines was investigated. For time-dependent studies, three cell types were stimulated with 10ng/ml IL-1β or 50ng/ml TNF-α for 8, 16, and 24h. For concentration-dependent studies, chondrocytes were stimulated with a higher level of IL-1β (20ng/ml) or TNF-α (100ng/ml) for 8h. Cellular and extracellular expressions of miR-22, miR-16, miR-146a, miR-27b, and miR-140 were analyzed by RT-PCR. Time-dependent cellular miRNA expressions were similar across the three cell types with miR-146a significantly up-regulated and miR-27b significantly down-regulated at all time points. However, chondrocytes exhibited a unique extracellular miRNA profile with an increased release rate of miR-27b at 24h. Our findings support further research into the characterization of miRNAs in synovial fluid for the development of early detection strategies of OA or cartilage injury.

AB - In knee osteoarthritis (OA), concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α increase in joint tissues and synovial fluid which incite a catabolic cascade and further the progression of OA. Several microRNAs (miRNA) have been associated with apoptosis (miR-16), inflammation (miR-22, miR-146a), and matrix degradation (miR-140, miR-27b) in developed OA or its symptoms. In this study, the time- and concentration-dependent nature of cellular and extracellular miRNAs in synoviocytes, meniscus cells, and chondrocytes as influenced by inflammatory cytokines was investigated. For time-dependent studies, three cell types were stimulated with 10ng/ml IL-1β or 50ng/ml TNF-α for 8, 16, and 24h. For concentration-dependent studies, chondrocytes were stimulated with a higher level of IL-1β (20ng/ml) or TNF-α (100ng/ml) for 8h. Cellular and extracellular expressions of miR-22, miR-16, miR-146a, miR-27b, and miR-140 were analyzed by RT-PCR. Time-dependent cellular miRNA expressions were similar across the three cell types with miR-146a significantly up-regulated and miR-27b significantly down-regulated at all time points. However, chondrocytes exhibited a unique extracellular miRNA profile with an increased release rate of miR-27b at 24h. Our findings support further research into the characterization of miRNAs in synovial fluid for the development of early detection strategies of OA or cartilage injury.

KW - Chondrocytes

KW - Inflammatory cytokines

KW - MicroRNA

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