Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke

Nadine Kerr, Marta García-Contreras, Sam Abbassi, Nancy H. Mejias, Brandon R. Desousa, Camillo Ricordi, W. Dalton Dietrich, Robert W. Keane, Juan Pablo de Rivero Vaccari

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The inflammasome is a key contributor to the inflammatory innate immune response after stroke. We have previously shown that inflammasome proteins are released in extracellular vesicles (EV) after brain and spinal cord injury. In addition, we have shown that inflammasome proteins offer great promise as biomarkers of central nervous system (CNS) injury following brain trauma. In the present study, we used a Simple Plex Assay (Protein Simple), a novel multi-analyte automated microfluidic immunoassay platform, to analyze serum and serum-derived EV samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), Interleukins (IL)-1β, and (IL)-18. Receiver operator characteristic (ROC) curves with associated confidence intervals obtained from the analysis of serum samples revealed that the area under the curve (AUC) for ASC was 0.99 with a confidence interval between 0.9914 and 1.004, whereas the AUC for caspase-1, IL-1β, and IL-18 were 0.75, 0.61, and 0.67, respectively. Thus, these data indicate that ASC is a potential biomarker of stroke and highlight the role of the inflammasome in the inflammatory response after brain ischemia.

Original languageEnglish (US)
Article number309
JournalFrontiers in Molecular Neuroscience
Volume11
DOIs
StatePublished - Sep 4 2018

Keywords

  • ASC
  • Biomarkers
  • Caspase-1
  • Exosomes
  • Extracellular vesicles
  • Inflammasome
  • Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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