TY - JOUR
T1 - Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke
AU - Kerr, Nadine
AU - García-Contreras, Marta
AU - Abbassi, Sam
AU - Mejias, Nancy H.
AU - Desousa, Brandon R.
AU - Ricordi, Camillo
AU - Dietrich, W. Dalton
AU - Keane, Robert W.
AU - de Rivero Vaccari, Juan Pablo
N1 - Funding Information:
This work was supported by a grant to JdRV from the American Heart Association (12SDG11970010) and The Miami Project to Cure Paralysis and a STTR grant (4R42NS086274-012) from the NINDS/NIH to RK and WD.
Publisher Copyright:
© 2018 Kerr, García-Contreras, Abbassi, Mejias, Desousa, Ricordi, Dietrich, Keane and de Rivero Vaccari.
PY - 2018/9/4
Y1 - 2018/9/4
N2 - The inflammasome is a key contributor to the inflammatory innate immune response after stroke. We have previously shown that inflammasome proteins are released in extracellular vesicles (EV) after brain and spinal cord injury. In addition, we have shown that inflammasome proteins offer great promise as biomarkers of central nervous system (CNS) injury following brain trauma. In the present study, we used a Simple Plex Assay (Protein Simple), a novel multi-analyte automated microfluidic immunoassay platform, to analyze serum and serum-derived EV samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), Interleukins (IL)-1β, and (IL)-18. Receiver operator characteristic (ROC) curves with associated confidence intervals obtained from the analysis of serum samples revealed that the area under the curve (AUC) for ASC was 0.99 with a confidence interval between 0.9914 and 1.004, whereas the AUC for caspase-1, IL-1β, and IL-18 were 0.75, 0.61, and 0.67, respectively. Thus, these data indicate that ASC is a potential biomarker of stroke and highlight the role of the inflammasome in the inflammatory response after brain ischemia.
AB - The inflammasome is a key contributor to the inflammatory innate immune response after stroke. We have previously shown that inflammasome proteins are released in extracellular vesicles (EV) after brain and spinal cord injury. In addition, we have shown that inflammasome proteins offer great promise as biomarkers of central nervous system (CNS) injury following brain trauma. In the present study, we used a Simple Plex Assay (Protein Simple), a novel multi-analyte automated microfluidic immunoassay platform, to analyze serum and serum-derived EV samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), Interleukins (IL)-1β, and (IL)-18. Receiver operator characteristic (ROC) curves with associated confidence intervals obtained from the analysis of serum samples revealed that the area under the curve (AUC) for ASC was 0.99 with a confidence interval between 0.9914 and 1.004, whereas the AUC for caspase-1, IL-1β, and IL-18 were 0.75, 0.61, and 0.67, respectively. Thus, these data indicate that ASC is a potential biomarker of stroke and highlight the role of the inflammasome in the inflammatory response after brain ischemia.
KW - ASC
KW - Biomarkers
KW - Caspase-1
KW - Exosomes
KW - Extracellular vesicles
KW - Inflammasome
KW - Serum
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U2 - 10.3389/fnmol.2018.00309
DO - 10.3389/fnmol.2018.00309
M3 - Article
AN - SCOPUS:85054793493
VL - 11
JO - Frontiers in Molecular Neuroscience
JF - Frontiers in Molecular Neuroscience
SN - 1662-5099
M1 - 309
ER -