Inflammasome proteins as biomarkers of traumatic brain injury

Nadine Kerr, Stephanie W. Lee, Jon Perez-Barcena, Catalina Crespi, Javier Ibañez, M. Ross Bullock, W. Dalton Dietrich, Robert Keane, Juan Pablo de Rivero Vaccari

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background The inflammasome plays an important role in the inflammatory innate immune response after central nervous system (CNS) injury. Inhibition of the inflammasome after traumatic brain injury (TBI) results in improved outcomes by lowering the levels of caspase-1 and interleukin (IL)-1b. We have previously shown that inflammasome proteins are elevated in the cerebrospinal fluid (CSF) of patients with TBI and that higher levels of these proteins were consistent with poorer outcomes after TBI when compared to patients that presented these inflammasome proteins at lower levels. Methods and findings Here we extend our work by analyzing serum from 21 TBI patients and CSF from 18 TBI patients compared to 120 serum samples and 30 CSF samples from no-TBI donor controls for the expression of caspase-1, apoptosis-associated speck-like protein containing a cas-pase recruitment domain (ASC), interleukin(IL)-1b and IL-18. Analysis was carried out using the Ella Simple Plex system (Protein Simple) to determine the sensitivity and specificity of inflammasome proteins as biomarkers of TBI. Receiver operator characteristic (ROC) curves, confidence intervals and likelihood ratios for each biomarker was determined. ROC curves, confidence intervals, sensitivity and specificity for each biomarker examined revealed that caspase-1 (0.93 area under the curve (AUC)) and ASC (0.90 AUC) in serum and ASC (1.0 AUC) and IL-18 (0.84 AUC) in CSF are promising biomarkers of TBI pathology. Importantly, higher protein levels (above 547.6 pg/ml) of ASC (0.91 AUC) were consistent with poorer outcomes after TBI as determined by the Glasgow Outcome Scale-Extended (GOSE).

Original languageEnglish (US)
Article numbere0210128
JournalPLoS One
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2018

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Inflammasomes
Biomarkers
biomarkers
Brain
brain
Cerebrospinal fluid
Area Under Curve
cerebrospinal fluid
caspase-1
Caspase 1
Proteins
Cerebrospinal Fluid
proteins
interleukin-18
Interleukin-18
Interleukins
interleukins
confidence interval
simple proteins
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kerr, N., Lee, S. W., Perez-Barcena, J., Crespi, C., Ibañez, J., Ross Bullock, M., ... Pablo de Rivero Vaccari, J. (2018). Inflammasome proteins as biomarkers of traumatic brain injury. PLoS One, 13(12), [e0210128]. https://doi.org/10.1371/journal.pone.0210128

Inflammasome proteins as biomarkers of traumatic brain injury. / Kerr, Nadine; Lee, Stephanie W.; Perez-Barcena, Jon; Crespi, Catalina; Ibañez, Javier; Ross Bullock, M.; Dalton Dietrich, W.; Keane, Robert; Pablo de Rivero Vaccari, Juan.

In: PLoS One, Vol. 13, No. 12, e0210128, 01.12.2018.

Research output: Contribution to journalArticle

Kerr, N, Lee, SW, Perez-Barcena, J, Crespi, C, Ibañez, J, Ross Bullock, M, Dalton Dietrich, W, Keane, R & Pablo de Rivero Vaccari, J 2018, 'Inflammasome proteins as biomarkers of traumatic brain injury', PLoS One, vol. 13, no. 12, e0210128. https://doi.org/10.1371/journal.pone.0210128
Kerr N, Lee SW, Perez-Barcena J, Crespi C, Ibañez J, Ross Bullock M et al. Inflammasome proteins as biomarkers of traumatic brain injury. PLoS One. 2018 Dec 1;13(12). e0210128. https://doi.org/10.1371/journal.pone.0210128
Kerr, Nadine ; Lee, Stephanie W. ; Perez-Barcena, Jon ; Crespi, Catalina ; Ibañez, Javier ; Ross Bullock, M. ; Dalton Dietrich, W. ; Keane, Robert ; Pablo de Rivero Vaccari, Juan. / Inflammasome proteins as biomarkers of traumatic brain injury. In: PLoS One. 2018 ; Vol. 13, No. 12.
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