Induction of the Yersinia Type 3 Secretion System as an All-or-None Phenomenon

David J. Wiley, Roland Rosqvist, Kurt Schesser

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Pathogenic Yersinia spp. possess a protein secretion system, designated as type 3, that plays a clear role in promoting their survival vis-à-vis the macrophage. Inductive expression of the Yersinia type 3 secretion system (T3SS), triggered either by host cell contact, or, in the absence of host cells, by a reduction in extracellular calcium ion levels, is accompanied by a withdrawal from the bacterial division cycle. Here, we analyzed Ca2+-dependent induction of the T3SS at the single-cell level to understand how Yersinia coordinates pro-survival and growth-related activities. We utilized a novel high-throughput quantitative microscopy approach as well as flow cytometry to determine how Ca2+ levels, T3SS expression, and cellular division are interrelated. Our analysis showed that there is a high degree of homogeneity in terms of T3SS expression levels among a population of Y. pseudotuberculosis cells following the removal of Ca2+, and that T3SS expression appears to be independent of the cellular division cycle. Unexpectedly, our analysis showed that Ca2+ levels are inversely related to the initiation of inductive T3SS expression, and not to the intensity of activation once initiated, thus providing a basis for the seemingly graded response of T3SS activation observed in bulk-level analyses. The properties of the system described here display both similarities to and differences from that of the lac operon first described 50 years ago by Novick and Weiner.

Original languageEnglish
Pages (from-to)27-37
Number of pages11
JournalJournal of Molecular Biology
Volume373
Issue number1
DOIs
StatePublished - Oct 12 2007

Fingerprint

Yersinia
Lac Operon
Type III Secretion Systems
Microscopy
Flow Cytometry
Macrophages
Ions
Calcium
Growth
Population

Keywords

  • gene expression
  • induction
  • type 3 secretion
  • Yersinia
  • Yops

ASJC Scopus subject areas

  • Virology

Cite this

Induction of the Yersinia Type 3 Secretion System as an All-or-None Phenomenon. / Wiley, David J.; Rosqvist, Roland; Schesser, Kurt.

In: Journal of Molecular Biology, Vol. 373, No. 1, 12.10.2007, p. 27-37.

Research output: Contribution to journalArticle

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