Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA

Smita K. Nair, David Boczkowski, Michael Morse, R. Ian Cumming, H. Kim Lyerly, Eli Gilboa

Research output: Contribution to journalArticlepeer-review

361 Scopus citations

Abstract

Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be necessary to generate and/or maintain an optimal CD8+ CTL response in vivo. CEA RNA-transfected DCs also serve as effective targets in cytotoxicity assays, thus providing a general method for inducing, as well as measuring, CEA-specific CTL responses across a broad spectrum of HLA haplotypes.

Original languageEnglish (US)
Pages (from-to)364-369
Number of pages6
JournalNature Biotechnology
Volume16
Issue number4
DOIs
StatePublished - Apr 1 1998
Externally publishedYes

Keywords

  • Antigen presenting cells
  • Applied immunology
  • Cancer vaccine

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA'. Together they form a unique fingerprint.

Cite this