Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA

Smita K. Nair, David Boczkowski, Michael Morse, R. Ian Cumming, H. Kim Lyerly, Eli Gilboa

Research output: Contribution to journalArticle

361 Scopus citations

Abstract

Dendritic cells (DC) generated from the peripheral blood mononuclear cells of healthy individuals or from cancer patients transfected with carcinoembryonic antigen (CEA) mRNA stimulate a potent CD8+ cytotoxic T lymphocyte (CTL) response in vitro. DCs are effectively sensitized with RNA in the absence of reagents commonly used to facilitate mammalian cell transfection. RNA encoding a chimeric CEA/LAMP-1 lysosomal targeting signal enhances the induction of CEA-specific CD4+ T cells, providing a strategy to induce T-help that may be necessary to generate and/or maintain an optimal CD8+ CTL response in vivo. CEA RNA-transfected DCs also serve as effective targets in cytotoxicity assays, thus providing a general method for inducing, as well as measuring, CEA-specific CTL responses across a broad spectrum of HLA haplotypes.

Original languageEnglish (US)
Pages (from-to)364-369
Number of pages6
JournalNature Biotechnology
Volume16
Issue number4
DOIs
StatePublished - Apr 1 1998
Externally publishedYes

Keywords

  • Antigen presenting cells
  • Applied immunology
  • Cancer vaccine

ASJC Scopus subject areas

  • Microbiology

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