Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA

B. D. Foy, T. Magalhaes, W. E. Injera, I. Sutherland, M. Devenport, A. Thanawastien, D. Ripley, L. Cárdenas-Freytag, John C Beier

Research output: Contribution to journalArticle

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Abstract

Vaccines that induce mosquito-killing (mosquitocidal) activity could substantially reduce the transmission of certain mosquito-borne diseases, especially vaccines against African malaria vectors, such as the mosquito Anopheles gambiae. To generate and characterize antimosquito immunity we immunized groups of mice with two individual A. gambiae midgut cDNAs, Ag-Aper1 (a secreted peritrophic matrix protein) and AgMuc1 (a midgut-bound mucin), and an A. gambiae midgut cDNA library from blood-fed mosquitoes. We observed significantly increased mortality among mosquitoes that fed on either the AgMuc1- or the cDNA library-immunized mice compared to that of controls, but no differences were observed among those fed on Ag-Aper1-immunized mice. Analysis of the humoral and cellular immune responses from mice showed that the induced mosquitocidal effect was associated with immune profiles characterized by elevated tumor necrosis factor alpha and gamma interferon cytokine levels and very low antibody titers. Furthermore, an additional immunization of cDNA library-immunized mice with midgut protein shifted immunity toward a Th2-type immune response, characterized by elevated antibody titers and high interleukin-5 and interleukin-10 cytokine levels; importantly, mosquitoes feeding on these mice exhibited no undo mortality. Finally, when immune sera was ingested by mosquitoes through a membrane feeder, no effect on mosquito mortality was observed, indicating that serum factors alone were not responsible for the mosquitocidal effect. Our results demonstrate that mosquitocidal immunity in mice can be consistently generated by midgut cDNA immunization and suggest this cDNA-induced mosquitocidal immunity is cell mediated.

Original languageEnglish
Pages (from-to)2032-2040
Number of pages9
JournalInfection and Immunity
Volume71
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

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Anopheles gambiae
Culicidae
Complementary DNA
Gene Library
Immunity
Cellular Immunity
Mortality
Immunization
Vaccines
Cytokines
Antibodies
Interleukin-5
Mucins
Humoral Immunity
Interferon-alpha
Interleukin-10
Malaria
Interferon-gamma
Immune Sera
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Foy, B. D., Magalhaes, T., Injera, W. E., Sutherland, I., Devenport, M., Thanawastien, A., ... Beier, J. C. (2003). Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA. Infection and Immunity, 71(4), 2032-2040. https://doi.org/10.1128/IAI.71.4.2032-2040.2003

Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA. / Foy, B. D.; Magalhaes, T.; Injera, W. E.; Sutherland, I.; Devenport, M.; Thanawastien, A.; Ripley, D.; Cárdenas-Freytag, L.; Beier, John C.

In: Infection and Immunity, Vol. 71, No. 4, 01.04.2003, p. 2032-2040.

Research output: Contribution to journalArticle

Foy, BD, Magalhaes, T, Injera, WE, Sutherland, I, Devenport, M, Thanawastien, A, Ripley, D, Cárdenas-Freytag, L & Beier, JC 2003, 'Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA', Infection and Immunity, vol. 71, no. 4, pp. 2032-2040. https://doi.org/10.1128/IAI.71.4.2032-2040.2003
Foy BD, Magalhaes T, Injera WE, Sutherland I, Devenport M, Thanawastien A et al. Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA. Infection and Immunity. 2003 Apr 1;71(4):2032-2040. https://doi.org/10.1128/IAI.71.4.2032-2040.2003
Foy, B. D. ; Magalhaes, T. ; Injera, W. E. ; Sutherland, I. ; Devenport, M. ; Thanawastien, A. ; Ripley, D. ; Cárdenas-Freytag, L. ; Beier, John C. / Induction of mosquitocidal activity in mice immunized with Anopheles gambiae midgut cDNA. In: Infection and Immunity. 2003 ; Vol. 71, No. 4. pp. 2032-2040.
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