Induction of methemoglobin formation by nitrosochloramphenicol in hemolyzed blood

L. O. Lim, Adel A Yunis

Research output: Contribution to journalArticle

Abstract

We studied the effects of chloramphenicol (CAP), nitroso-chloramphenicol (NO-CAP) and thiamphenicol (TAP) on methemoglobin (MeHb) formation in vitro in hemolyzed blood. At concentrations of 0.5 to 10 mM CAP and TAP did not have any effect on hemoglobin. On the other hand, NO-CAP at 0.25 to 5 mM caused both time and concentration dependent increase in MeHb formation. The oxidation of hemoglobin was reversible by potassium cyanide. Since MeHb was detected only at NO-CAP concentration 0.25 nM or higher, methemoglobin formation is not a sensitive indication of NO-CAP presence.

Original languageEnglish (US)
Pages (from-to)497-500
Number of pages4
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume38
Issue number3
StatePublished - 1982

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Methemoglobin
Chloramphenicol
Blood
Thiamphenicol
Hemoglobins
Potassium Cyanide
nitrosochloramphenicol
Oxidation

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Induction of methemoglobin formation by nitrosochloramphenicol in hemolyzed blood. / Lim, L. O.; Yunis, Adel A.

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 38, No. 3, 1982, p. 497-500.

Research output: Contribution to journalArticle

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