Induction of methemoglobin formation by nitrosochloramphenicol in hemolyzed blood

L. O. Lim, A. A. Yunis

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We studied the effects of chloramphenicol (CAP), nitroso-chloramphenicol (NO-CAP) and thiamphenicol (TAP) on methemoglobin (MeHb) formation in vitro in hemolyzed blood. At concentrations of 0.5 to 10 mM CAP and TAP did not have any effect on hemoglobin. On the other hand, NO-CAP at 0.25 to 5 mM caused both time and concentration dependent increase in MeHb formation. The oxidation of hemoglobin was reversible by potassium cyanide. Since MeHb was detected only at NO-CAP concentration 0.25 nM or higher, methemoglobin formation is not a sensitive indication of NO-CAP presence.

Original languageEnglish (US)
Pages (from-to)497-500
Number of pages4
JournalResearch Communications in Chemical Pathology and Pharmacology
Issue number3
StatePublished - Dec 1 1982

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)


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