Abstract
We studied the effects of chloramphenicol (CAP), nitroso-chloramphenicol (NO-CAP) and thiamphenicol (TAP) on methemoglobin (MeHb) formation in vitro in hemolyzed blood. At concentrations of 0.5 to 10 mM CAP and TAP did not have any effect on hemoglobin. On the other hand, NO-CAP at 0.25 to 5 mM caused both time and concentration dependent increase in MeHb formation. The oxidation of hemoglobin was reversible by potassium cyanide. Since MeHb was detected only at NO-CAP concentration 0.25 nM or higher, methemoglobin formation is not a sensitive indication of NO-CAP presence.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 497-500 |
| Number of pages | 4 |
| Journal | Research Communications in Chemical Pathology and Pharmacology |
| Volume | 38 |
| Issue number | 3 |
| State | Published - 1982 |
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ASJC Scopus subject areas
- Pharmacology
- Toxicology
Cite this
Induction of methemoglobin formation by nitrosochloramphenicol in hemolyzed blood. / Lim, L. O.; Yunis, Adel A.
In: Research Communications in Chemical Pathology and Pharmacology, Vol. 38, No. 3, 1982, p. 497-500.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of methemoglobin formation by nitrosochloramphenicol in hemolyzed blood
AU - Lim, L. O.
AU - Yunis, Adel A
PY - 1982
Y1 - 1982
N2 - We studied the effects of chloramphenicol (CAP), nitroso-chloramphenicol (NO-CAP) and thiamphenicol (TAP) on methemoglobin (MeHb) formation in vitro in hemolyzed blood. At concentrations of 0.5 to 10 mM CAP and TAP did not have any effect on hemoglobin. On the other hand, NO-CAP at 0.25 to 5 mM caused both time and concentration dependent increase in MeHb formation. The oxidation of hemoglobin was reversible by potassium cyanide. Since MeHb was detected only at NO-CAP concentration 0.25 nM or higher, methemoglobin formation is not a sensitive indication of NO-CAP presence.
AB - We studied the effects of chloramphenicol (CAP), nitroso-chloramphenicol (NO-CAP) and thiamphenicol (TAP) on methemoglobin (MeHb) formation in vitro in hemolyzed blood. At concentrations of 0.5 to 10 mM CAP and TAP did not have any effect on hemoglobin. On the other hand, NO-CAP at 0.25 to 5 mM caused both time and concentration dependent increase in MeHb formation. The oxidation of hemoglobin was reversible by potassium cyanide. Since MeHb was detected only at NO-CAP concentration 0.25 nM or higher, methemoglobin formation is not a sensitive indication of NO-CAP presence.
UR - http://www.scopus.com/inward/record.url?scp=0020366855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020366855&partnerID=8YFLogxK
M3 - Article
VL - 38
SP - 497
EP - 500
JO - Research Communications in Molecular Pathology and Pharmacology
JF - Research Communications in Molecular Pathology and Pharmacology
SN - 0034-5164
IS - 3
ER -