Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239

Vito G. Sasseville, Zhenjian Du, Laura V. Chalifoux, Douglas R. Pauley, Heather L. Young, Prabhat K. Sehgal, Ronald Charles Desrosiers, Andrew A. Lackner

Research output: Contribution to journalArticle

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Abstract

The molecularly cloned virus known as SIV-mac239/YEnef causes extensive lymphocyte activation in unstimulated peripheral blood mononuclear cell cultures and induces an acute disease syndrome in macaque monkeys. Here we describe the histopathological and immunophenotypic changes and viral localization in peripheral lymph nodes, spleen, and gastrointestinal tract (including the gut-associated lymphoid tissue (GALT)) in rhesus monkeys inoculated with SIVmac239/YEnef beginning at day 3 postinoculation (pi). The findings are compared with those of rhesus monkeys inoculated with the same dose of parental SiVmac239. Histopathological examination of peripheral lymphoid tissue and GALT demonstrated marked hyperplasia of T-cell-dependent regions and involution of germinal centers as early as day 7 pi. The most striking lesions were multifocal areas of lymphohistiocytic gastroenteritis and colitis. Cellular infiltrates peaked between day 7 and 14 pi and were composed primarily of CD3+ T lymphocytes and HAM·56+ monocyte/macrophages. Many of these inflammatory cells were also strongly immunoreactive for the nuclear proliferation antigen Ki-67. Despite the presence of severe gastrointestinal pathology by day 7 pi, no significant difference in the numbers of virus-positive cells in the gastrointestinal tract was observed between these animals and SIVmac239-infected animals examined at the same time point. However, the distribution of virus in the gastrointestinal tract was markedly different, with virus localized to lymphoid nodules of GALT in SIVmac239-infected animals and restricted to areas of lymphohistiocytic gastroenteritis and colitis in animals infected with SIVmac239/YEnef. Our data indicate that the acute disease syndrome induced by SIVmac239/YEnef is not simply related to increased vital replication in the gastrointestinal tract but is likely due to inappropriate virus-induced T lymphocyte activation and proliferation in GALT and subsequent mucosal destruction.

Original languageEnglish (US)
Pages (from-to)163-176
Number of pages14
JournalAmerican Journal of Pathology
Volume149
Issue number1
StatePublished - Jul 1996
Externally publishedYes

Fingerprint

nef Genes
Gastrointestinal Diseases
Macaca
Lymphoid Tissue
Lymphocytes
Gastrointestinal Tract
Viruses
Gastroenteritis
Acute Disease
Colitis
Lymphocyte Activation
Macaca mulatta
T-Lymphocytes
Nuclear Antigens
Germinal Center
Hyperplasia
Haplorhini
Monocytes
Blood Cells
Spleen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sasseville, V. G., Du, Z., Chalifoux, L. V., Pauley, D. R., Young, H. L., Sehgal, P. K., ... Lackner, A. A. (1996). Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239. American Journal of Pathology, 149(1), 163-176.

Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239. / Sasseville, Vito G.; Du, Zhenjian; Chalifoux, Laura V.; Pauley, Douglas R.; Young, Heather L.; Sehgal, Prabhat K.; Desrosiers, Ronald Charles; Lackner, Andrew A.

In: American Journal of Pathology, Vol. 149, No. 1, 07.1996, p. 163-176.

Research output: Contribution to journalArticle

Sasseville, VG, Du, Z, Chalifoux, LV, Pauley, DR, Young, HL, Sehgal, PK, Desrosiers, RC & Lackner, AA 1996, 'Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239', American Journal of Pathology, vol. 149, no. 1, pp. 163-176.
Sasseville VG, Du Z, Chalifoux LV, Pauley DR, Young HL, Sehgal PK et al. Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239. American Journal of Pathology. 1996 Jul;149(1):163-176.
Sasseville, Vito G. ; Du, Zhenjian ; Chalifoux, Laura V. ; Pauley, Douglas R. ; Young, Heather L. ; Sehgal, Prabhat K. ; Desrosiers, Ronald Charles ; Lackner, Andrew A. / Induction of lymphocyte proliferation and severe gastrointestinal disease in macaques by a nef gene variant of SIVmac239. In: American Journal of Pathology. 1996 ; Vol. 149, No. 1. pp. 163-176.
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