Induction of ischemic stroke and ischemia-reperfusion in mice using the middle artery occlusion technique and visualization of infarct area

Luc Bertrand, Levi Dygert, Michal J Toborek

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Cerebrovascular disease is highly prevalent in the global population and encompasses several types of conditions, including stroke. To study the impact of stroke on tissue injury and to evaluate the effectiveness of therapeutic interventions, several experimental models in a variety of species were developed. They include complete global cerebral ischemia, incomplete global ischemia, focal cerebral ischemia, and multifocal cerebral ischemia. The model described in this protocol is based on the middle cerebral artery occlusion (MCAO) and is related to the focal ischemia category. This technique produces consistent focal ischemia in a strictly defined region of the hemisphere and is less invasive than other methods. The procedure described is performed on mice, given the availability of several genetic variants and the high number of tests standardized for mice to aid in the behavioral and neurodeficit evaluation.

Original languageEnglish (US)
Article numbere54805
JournalJournal of Visualized Experiments
Volume2017
Issue number120
DOIs
StatePublished - Feb 22 2017

Keywords

  • Ischemia
  • Issue 120
  • MCAO
  • Medicine
  • Microsurgery
  • Mouse
  • Stroke
  • Tissue imaging

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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