Induction of intercellular adhesion molecule-1 (CD54) on isolated mouse pancreatic β cells by inflammatory cytokines

Jacqueline Prieto, Ephata E. Kaaya, Lisa Juntti-Berggren, Per Olof Berggren, Stellan Sandler, Peter Biberfeld, Manuel Patarroyo

Research output: Contribution to journalArticle

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Abstract

Insulin-dependent diabetes mellitus (IDDM) results from a T cell-dependent autoimmune destruction of insulin-producing pancreatic β cells. In the present study, expression of adhesion molecule ICAM-1 (CD54) on pancreatic β cells was studied in normal, obese hyperglycemic (ob/ob), and nonobese diabetic (NOD) mice. Freshly isolated pancreatic β cells from ob/ob mice did not express ICAM-1, but treatment of the cells with IL-1-β, TNF-α, or INF-γ strongly induced its expression as measured by immunofluorescence flow cytometry. The cytokines acted in a dose- and time-dependent manner. Maximal induction by either cytokine occurred at 24 hr and thereafter expression decreased, except for INF-γ. Immunoprecipitation from IL-1-β-treated β cells demonstrated a cell-surface glycoprotein with an apparent molecular weight of 95 kDa. ICAM-1 expression was undetectable on pancreatic β cells of normal and ob/ob mice as measured by immunohistochemistry. In NOD mice at different ages (1 to 6 months) ICAM-1 was also undetectable on β cells, in contrast to the strong expression on infiltrating mononuclear cells. The present study indicates that mouse pancreatic β cells, under certain conditions, can express ICAM-1.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalClinical Immunology and Immunopathology
Volume65
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Intercellular Adhesion Molecule-1
Cytokines
Inbred NOD Mouse
Interleukin-1
Membrane Glycoproteins
Type 1 Diabetes Mellitus
Immunoprecipitation
Fluorescent Antibody Technique
Flow Cytometry
Molecular Weight
Immunohistochemistry
Insulin
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Prieto, J., Kaaya, E. E., Juntti-Berggren, L., Berggren, P. O., Sandler, S., Biberfeld, P., & Patarroyo, M. (1992). Induction of intercellular adhesion molecule-1 (CD54) on isolated mouse pancreatic β cells by inflammatory cytokines. Clinical Immunology and Immunopathology, 65(3), 247-253. https://doi.org/10.1016/0090-1229(92)90154-G

Induction of intercellular adhesion molecule-1 (CD54) on isolated mouse pancreatic β cells by inflammatory cytokines. / Prieto, Jacqueline; Kaaya, Ephata E.; Juntti-Berggren, Lisa; Berggren, Per Olof; Sandler, Stellan; Biberfeld, Peter; Patarroyo, Manuel.

In: Clinical Immunology and Immunopathology, Vol. 65, No. 3, 01.01.1992, p. 247-253.

Research output: Contribution to journalArticle

Prieto, J, Kaaya, EE, Juntti-Berggren, L, Berggren, PO, Sandler, S, Biberfeld, P & Patarroyo, M 1992, 'Induction of intercellular adhesion molecule-1 (CD54) on isolated mouse pancreatic β cells by inflammatory cytokines', Clinical Immunology and Immunopathology, vol. 65, no. 3, pp. 247-253. https://doi.org/10.1016/0090-1229(92)90154-G
Prieto, Jacqueline ; Kaaya, Ephata E. ; Juntti-Berggren, Lisa ; Berggren, Per Olof ; Sandler, Stellan ; Biberfeld, Peter ; Patarroyo, Manuel. / Induction of intercellular adhesion molecule-1 (CD54) on isolated mouse pancreatic β cells by inflammatory cytokines. In: Clinical Immunology and Immunopathology. 1992 ; Vol. 65, No. 3. pp. 247-253.
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