Induction of il21 in peripheral t follicular helper cells is an indicator of influenza vaccine response in a previously vaccinated HIV-infected pediatric cohort

Lesley R. De Armas, Nicola Cotugno, Suresh Pallikkuth, Li Pan, Stefano Rinaldi, M. Celeste Sanchez, Louis Gonzalez, Alberto Cagigi, Paolo Rossi, Paolo Palma, Savita G Pahwa

Research output: Contribution to journalArticle

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Abstract

HIV-infected patients of all ages frequently underperform in response to seasonal influenza vaccination, despite virologic control of HIV. The molecular mechanisms governing this impairment, as well as predictive biomarkers for responsiveness, remain unknown. This study was performed in samples obtained prevaccination (T0) from HIV-infected children who received the 2012-2013 seasonal influenza vaccine. Response status was determined based on established criterion for hemagglutination inhibition titer; participants with a hemagglutination titer 1:40 plus a 4-fold increase over T0 at 3 wk postvaccination were designated as responders. All children had a history of prior influenza vaccinations. At T0, the frequencies of CD4 T cell subsets, including peripheral T follicular helper (pTfh) cells, which provide help to B cells for developing into Ab-secreting cells, were similar between responders and nonresponders. However, in response to in vitro stimulation with influenza A/California/7/2009 (H1N1) Ag, differential gene expression related to pTfh cell function was observed by Fluidigm high-density RT-PCR between responders and nonresponders. In responders, H1N1 stimulation at T0 also resulted in CXCR5 induction (mRNA and protein) in CD4 T cells and IL21 gene induction in pTfh cells that were strongly associated with H1N1-specific B cell responses postvaccination. In contrast, CD4 T cells of nonresponders exhibited increased expression of IL2 and STAT5 genes, which are known to antagonize peripheral Tfh cell function. These results suggest that the quality of pTfh cells at the time of immunization is important for influenza vaccine responses and provide a rationale for targeted, ex vivo Ag-driven molecular profiling of purified immune cells to detect predictive biomarkers of the vaccine response.

Original languageEnglish (US)
Pages (from-to)1995-2005
Number of pages11
JournalJournal of Immunology
Volume198
Issue number5
DOIs
StatePublished - Mar 1 2017

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Influenza Vaccines
Helper-Inducer T-Lymphocytes
HIV
Pediatrics
Human Influenza
Hemagglutination
Vaccination
B-Lymphocytes
Biomarkers
T-Lymphocytes
T-Lymphocyte Subsets
Genes
Interleukin-2
Immunization
Vaccines
Gene Expression
Polymerase Chain Reaction
Messenger RNA
interleukin-21
Proteins

ASJC Scopus subject areas

  • Immunology

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Induction of il21 in peripheral t follicular helper cells is an indicator of influenza vaccine response in a previously vaccinated HIV-infected pediatric cohort. / De Armas, Lesley R.; Cotugno, Nicola; Pallikkuth, Suresh; Pan, Li; Rinaldi, Stefano; Sanchez, M. Celeste; Gonzalez, Louis; Cagigi, Alberto; Rossi, Paolo; Palma, Paolo; Pahwa, Savita G.

In: Journal of Immunology, Vol. 198, No. 5, 01.03.2017, p. 1995-2005.

Research output: Contribution to journalArticle

De Armas, Lesley R. ; Cotugno, Nicola ; Pallikkuth, Suresh ; Pan, Li ; Rinaldi, Stefano ; Sanchez, M. Celeste ; Gonzalez, Louis ; Cagigi, Alberto ; Rossi, Paolo ; Palma, Paolo ; Pahwa, Savita G. / Induction of il21 in peripheral t follicular helper cells is an indicator of influenza vaccine response in a previously vaccinated HIV-infected pediatric cohort. In: Journal of Immunology. 2017 ; Vol. 198, No. 5. pp. 1995-2005.
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AU - Gonzalez, Louis

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AU - Pahwa, Savita G

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