Induction of early growth response gene Egr2 by basic calcium phosphate crystals through a calcium-dependent protein kinase C-independent p44/42 mitogen-activated protein kinase pathway

Xiao R. Zeng, Yubo Sun, Leonor Wenger, Herman S. Cheung

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Using the reverse transcriptase-polymerase chain reaction we examined the effect of basic calcium phosphate (BCP) crystals on the induction of the early growth response gene Egr2 transcription and the signal transduction pathway involved. The results showed that BCP crystals induced Egr2 transcription up to 8-fold, peaking at 24 h after treatment. The induction of Egr2 was confirmed by transient transfection assays using an Egr2 promoter/luciferase reporter construct and could be inhibited by the p44/42 mitogen-activated protein kinase (MAPK)-specific inhibitor U0126, or by calcium chelator TMB-8, but not by the SAPK2/p38 MAPK inhibitor SB202190 or by the protein kinase C inhibitor bisindolylmaleimide I (Bis-I). Using the Mercury Pathway Profiling System (Clontech, Palo Alto, Calif., USA) we further showed that induced Egr2 could stimulate the activities of several transcription factors that are associated with cell proliferation, such as c-fos, SRF and c-myc.

Original languageEnglish (US)
Pages (from-to)63-72
Number of pages10
JournalCells Tissues Organs
Volume174
Issue number1-2
DOIs
StatePublished - Jun 17 2003

Keywords

  • Basic calcium phosphate crystals
  • Early growth response gene
  • Gene expression
  • Transcriptional factors

ASJC Scopus subject areas

  • Anatomy

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