Induction of cytotoxic T lymphocytes with dendritic cells transfected with human papillomavirus E6 and E7 RNA: Implications for cervical cancer immunotherapy

Courtney Thornburg, David Boczkowski, Eli Gilboa, Smita K. Nair

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Human papillomavirus (HPV) infection is associated with cervical cancer. The high-risk HPV E6 and E7 oncoproteins are constitutively expressed in most cervical carcinoma cells, and are, therefore, attractive antigens for cytotoxic T-lymphocyte (CTL)-mediated immunotherapy. The objective of this study was to evaluate the use of dendritic cells (DCs) transfected with RNA encoding the E6 and E7 protein for cervical cancer immunotherapy. The authors have shown that DCs transfected with RNA-encoding antigen stimulate potent antigen-specific CTL responses in vitro and in vivo. In this study, they tried to determine whether DCs transfected with E6 and E7 RNA stimulate primary, antigen-specific CTL responses in vitro. The results show that DCs pulsed with E6 or E7 RNA stimulate antigen-specific CTL responses that recognize and lyse DCs transfected with E6 and E7 RNA and human cervical carcinoma Cells expressing the E6 and E7 products, and the lysis was comparable to that achieved with E6 and E7 peptide-pulsed DCs. Dendritic cells cotransfected with both E6 and E7 RNA stimulate CTLs that are more effective at lysing human cervical cancer cells. This study provides a rationale for the development of cervical carcinoma immunotherapy using DCs transfected with HPV E6 and E7 RNA.

Original languageEnglish (US)
Pages (from-to)412-418
Number of pages7
JournalJournal of Immunotherapy
Volume23
Issue number4
DOIs
StatePublished - Jul 1 2000
Externally publishedYes

Keywords

  • Cancer Vaccine
  • Cervical Cancer
  • Dendritic Cells
  • Human Papillomavirus
  • RNA

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Immunology

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