Induction of conjunctival transdifferentiation on vacularized coneas by photothrombotic occlusion of corneal neovascularization

A. J W Huang, B. D. Watson, E. Hernandez, S. C G Tseng

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Abstract

Previous studies have established that conjunctival transdifferentiation (transformation into cornea-like morphology) is inhibited by corneal vascularization. Conversely, occlusion of corneal vessels may induce conjunctival transdifferentiation on vascularized corneas. To test this hypothesis, the corneal epithelia of New Zealand albino rabbits were debrided 3 mm beyond the limbus with n-heptanol. Sixteen corneas healed by conjunctival epitheliunm, with vascularization persisting for 20 months, were used in this study. Photochemically induced occlusion of the corneal vessels was achieved by intravenous administration of rose bengal-saline solution (40 mg/kg body weight) with subsequent argon laser irradiation of the vessels (514.5 nm, 130 mW, 63 μm and 0.2 sec). The treated vessels remained occluded in an 18-week study, as confirmed by corneal fluorescein angiography. Corneal clarity and epithelial integrity were improved after treatment. Goblet cell loss and morphologic transformation into a cornea-like epithelium were verified by flat-mount preparations, histology, impression cytology, and immunofluorescence studies using a mucin-specific monoclonal antibody. These results indicate that conjunctival transdifferentiation can be induced on vascularized corneas after occlusion of corneal vessels by photothrombosis.

Original languageEnglish
Pages (from-to)228-235
Number of pages8
JournalOphthalmology
Volume95
Issue number2
StatePublished - Jan 1 1988

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Corneal Neovascularization
Cornea
Heptanol
Rose Bengal
Corneal Epithelium
Goblet Cells
Fluorescein Angiography
Argon
Mucins
Sodium Chloride
Intravenous Administration
Fluorescent Antibody Technique
Cell Biology
Histology
Lasers
Epithelium
Monoclonal Antibodies
Body Weight
Rabbits

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Induction of conjunctival transdifferentiation on vacularized coneas by photothrombotic occlusion of corneal neovascularization. / Huang, A. J W; Watson, B. D.; Hernandez, E.; Tseng, S. C G.

In: Ophthalmology, Vol. 95, No. 2, 01.01.1988, p. 228-235.

Research output: Contribution to journalArticle

Huang, A. J W ; Watson, B. D. ; Hernandez, E. ; Tseng, S. C G. / Induction of conjunctival transdifferentiation on vacularized coneas by photothrombotic occlusion of corneal neovascularization. In: Ophthalmology. 1988 ; Vol. 95, No. 2. pp. 228-235.
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