TY - JOUR
T1 - Induction of cell-cell channel formation by mRNA
AU - Dahl, G.
AU - Azarnia, R.
AU - Werner, R.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1981
Y1 - 1981
N2 - Intercellular junctional communication is very common in normal organized tissue1. It provides a pathway for transmission of electrical signals, especially in heart muscle2,3, and may be important in differentiation and growth control1,4. The hydrophilic channels which enable cell-cell communication have been well characterized by biophysical methods5,6, and there is now good evidence that they are contained in the nexus (gap junctions)3,7-9. Little, however, is known about the molecular mechanism of biosynthesis of junctional channels. Knowledge in this area has been obtained almost exclusively from experiments with reaggregated cells10-15, a system complicated by the fact that de novo synthesis of channel proteins is obscured by reassembly of pre-existing subunits or utilization of precursors. To avoid these problems, we have now isolated mRNA from cells that are in the process of making new intercellular nexus with high efficiency, incorporated it via liposomes into communication-defective cells and have shown that the recipient cells established junctional communication.
AB - Intercellular junctional communication is very common in normal organized tissue1. It provides a pathway for transmission of electrical signals, especially in heart muscle2,3, and may be important in differentiation and growth control1,4. The hydrophilic channels which enable cell-cell communication have been well characterized by biophysical methods5,6, and there is now good evidence that they are contained in the nexus (gap junctions)3,7-9. Little, however, is known about the molecular mechanism of biosynthesis of junctional channels. Knowledge in this area has been obtained almost exclusively from experiments with reaggregated cells10-15, a system complicated by the fact that de novo synthesis of channel proteins is obscured by reassembly of pre-existing subunits or utilization of precursors. To avoid these problems, we have now isolated mRNA from cells that are in the process of making new intercellular nexus with high efficiency, incorporated it via liposomes into communication-defective cells and have shown that the recipient cells established junctional communication.
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U2 - 10.1038/289683a0
DO - 10.1038/289683a0
M3 - Article
C2 - 7464934
AN - SCOPUS:0019884193
VL - 289
SP - 683
EP - 685
JO - Nature
JF - Nature
SN - 0028-0836
IS - 5799
ER -