Induction of axon growth into schwann cell implants grafted into lesioned adult rat spinal cord

Carlos L. Paino; Mary Bartlett Bunge

doi:10.1016/0014-4886(91)90043-C

Induction of axon growth into schwann cell implants grafted into lesioned adult rat spinal cord

Carlos L. Paino, Mary Bartlett Bunge

Cell Biology

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Polymerized collagen rolls enclosing Schwann cells (SCs) raised in culture were grafted into cystic cavities formed after lesioning the thoracic spinal cord of adult rats. Axons were already present within the graft by 14 days after implantation and both ensheathed and myelinated axons were numerous by 28 days. This axonal ingrowth was maintained over longer survival periods. The axons within the graft always appeared related to Schwann cells. Acellular collagen rolls did not show axonal ingrowth. These Schwann cell-collagen implants resemble peripheral nerve grafts in their ability to induce axonal regeneration into the graft.

Original language	English (US)
Pages (from-to)	254-257
Number of pages	4
Journal	Experimental neurology
Volume	114
Issue number	2
DOIs	https://doi.org/10.1016/0014-4886(91)90043-C
State	Published - Nov 1991

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/0014-4886(91)90043-C

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title = "Induction of axon growth into schwann cell implants grafted into lesioned adult rat spinal cord",

abstract = "Polymerized collagen rolls enclosing Schwann cells (SCs) raised in culture were grafted into cystic cavities formed after lesioning the thoracic spinal cord of adult rats. Axons were already present within the graft by 14 days after implantation and both ensheathed and myelinated axons were numerous by 28 days. This axonal ingrowth was maintained over longer survival periods. The axons within the graft always appeared related to Schwann cells. Acellular collagen rolls did not show axonal ingrowth. These Schwann cell-collagen implants resemble peripheral nerve grafts in their ability to induce axonal regeneration into the graft.",

author = "Paino, {Carlos L.} and Bunge, {Mary Bartlett}",

note = "Funding Information: We thank Dr. Cristina Fernandez-Valle for contributions to cell culture and electron microscopy; Dr. Brant Watson for overseeing the use of the laser device; Dr. Vicky Holets, Ana Salvatierra, and Dr. Ricardo Prado for instruction in using the photothrombotic procedure; Margaret Bates, Judy Brown, and Joyce Olivier for expert laboratory assistance; Dr. Richard Bunge for discussion; and Charlaine Rowlette for word processing. This work was supported by NIH Grants NS15070, NS28059, NS09923; The Miami Project; and the Spanish Ministry of Education.",

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doi = "10.1016/0014-4886(91)90043-C",

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AU - Bunge, Mary Bartlett

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N2 - Polymerized collagen rolls enclosing Schwann cells (SCs) raised in culture were grafted into cystic cavities formed after lesioning the thoracic spinal cord of adult rats. Axons were already present within the graft by 14 days after implantation and both ensheathed and myelinated axons were numerous by 28 days. This axonal ingrowth was maintained over longer survival periods. The axons within the graft always appeared related to Schwann cells. Acellular collagen rolls did not show axonal ingrowth. These Schwann cell-collagen implants resemble peripheral nerve grafts in their ability to induce axonal regeneration into the graft.

AB - Polymerized collagen rolls enclosing Schwann cells (SCs) raised in culture were grafted into cystic cavities formed after lesioning the thoracic spinal cord of adult rats. Axons were already present within the graft by 14 days after implantation and both ensheathed and myelinated axons were numerous by 28 days. This axonal ingrowth was maintained over longer survival periods. The axons within the graft always appeared related to Schwann cells. Acellular collagen rolls did not show axonal ingrowth. These Schwann cell-collagen implants resemble peripheral nerve grafts in their ability to induce axonal regeneration into the graft.

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Induction of axon growth into schwann cell implants grafted into lesioned adult rat spinal cord

Abstract

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