Induction of antitumor immunity using bone marrow-generated dendritic cells

Angel Porgador, David Snyder, Eli Gilboa

Research output: Contribution to journalArticle

313 Scopus citations

Abstract

We have previously shown that bone marrow-generated dendritic cells (DC) pulsed with a class 1-restricted peptide are potent inducers of CD8+ CTL. In the present study we have investigated whether bone marrow-generated DC are capable of inducing antitumor immunity. We show that a single immunization with DC pulsed with OVA peptide was highly effective in eliciting a protective immune response against a challenge with tumor cells expressing the OVA gene (E.G7-OVA), more so than immunization with irradiated E.G7-OVA cells, OVA peptide-pulsed RMA-S cells, or free OVA peptide mixed with adjuvant. The addition of free OVA protein to day 4 or day 7 bone marrow cultures, but not to day 9 mature DC, was also effective in eliciting CTL and engendering antitumor immunity, but was less effective than peptide-pulsed DC. Induction of CTL and antitumor immunity by bone marrow-generated DC pulsed with the class I-restricted OVA peptide correlated with the expression of syngeneic MHC class II molecules on the DC. This and the fact that induction of tumor immunity was dependent on CD4+ T cells suggest that in vivo priming of CTL and induction of antitumor immunity by bone marrow-generated DC also require the presentation of MHC class II-restricted epitopes and activation of CD 4+ T cells. This observation has potentially important implications to the use of peptide-pulsed DC in clinical immunotherapy.

Original languageEnglish (US)
Pages (from-to)2918-2926
Number of pages9
JournalJournal of Immunology
Volume156
Issue number8
StatePublished - Apr 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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