Induced release and metabolism of arachidonic acid from myeloid cells by purified colony‐stimulating factor

Vincent A. Ziboh, Alan M. Miller, Ming‐Chi ‐C Wu, Adel A. Yunis, Joaquin Jimenez, Glen Wong

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14 Scopus citations


The in vitro incubation of cells from turpentine-induced rat myeloid hyperplastic marrow and peritoneal monocyte/macrophage with 14C-arachidonic acid resulted in the incorporation of the radiolabel into the particulate phospholipids. Challenge of the radiolabeled cells with a highly purified type 1 CSF (CSF 1) from human pancreatic carcinoma cells in continuous culture resulted in the hydrolysis and release of the 14C-arachidonic acid from the cellular phospholipids. The simultaneous challenge of the prelabeled cells with CSF-1 and its specific antibody (anti-CSF-1 antibody) inhibited the CSF-1 induced hydrolysis of 14C-arachidonic acid from the cells. These results confer a specificity on the CSF-1 induced release of arachidonic acid from the cellular phospholipids. Our data also demonstrated that the 14C-arachidonic acid released from the cellular phospholipids was further transformed into products of the cyclooxygenation and lipoxygenation pathways by cellular enzyme systems in both populations of cells. Interestingly, our data also indicate that the challenge of the granulocyte hyperplastic marrow cells and the monocyte/macrophage cells with purified CSF-1 resulted in a higher generation of lipoxygenase products in the predominantly granocytic cell population than in the population rich in monocyte/macrophage cells. The biological significance of this observation remains to be further explored. Thus the CSF-1-inducd release of cellular arachidonic acid explains, at least in part, the presence of prostaglandins and other metabolites of arachidonic acid that are found in the media of hemopoietic cells incubated with a variety of CSF preparations.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalJournal of Cellular Physiology
Issue number1
StatePublished - Oct 1982

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


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