Induced release and metabolism of arachidonic acid from myeloid cells by purified colony-stimulating factor

V. A. Ziboh, A. M. Miller, M. C. Wu, Adel A Yunis, Joaquin J Jimenez, G. Wong

Research output: Contribution to journalArticle

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Abstract

The in vitro incubation of cells from turpentine-induced rat myeloid hyperplastic marrow and peritoneal monocyte/macrophage with 14C-arachidonic acid resulted in the incorporation of the radiolabel into the particulate phospholipids. Challenge of the radiolabeled cells with a highly purified type 1 CSF (CSF 1) from human pancreatic carcinoma cells in continuous culture resulted in the hydrolysis and release of the 14C-arachidonic acid from the cellular phospholipids. The simultaneous challenge of the prelabeled cells with CSF-1 and its specific antibody (anti-CSF-1 antibody) inhibited the CSF-1 induced hydrolysis of 14C-arachidonic acid from the cells. These results confer a specificity on the CSF-1 induced release of arachidonic acid from the cellular phospholipids. Our data also demonstrated that the 14C-arachidonic acid released from the cellular phospholipids was further transformed into products of the cyclooxygenation and lipoxygenation pathways by cellular enzyme systems in both populations of cells. Interestingly, our data also indicate that the challenge of the granulocyte hyperplastic marrow cells and the monocyte/macrophage cells with purified CSF-1 resulted in a higher generation of lipoxygenase products in the predominantly granocytic cell population than in the population rich in monocyte/macrophage cells. The biological significance of this observation remains to be further explored. Thus the CSF-1-inducd release of cellular arachidonic acid explains, at least in part, the presence of prostaglandins and other metabolites of arachidonic acid that are found in the media of hemopoietic cells incubated with a variety of CSF preparations.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalJournal of Cellular Physiology
Volume113
Issue number1
DOIs
StatePublished - Dec 10 1982

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Colony-Stimulating Factors
Macrophage Colony-Stimulating Factor
Myeloid Cells
Arachidonic Acid
Metabolism
Cells
Macrophages
Phospholipids
Monocytes
Hydrolysis
Turpentine
Lipoxygenase
Bone Marrow
Metabolites
Population
Cell culture
Prostaglandins
Rats
Anti-Idiotypic Antibodies
Peritoneal Macrophages

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Induced release and metabolism of arachidonic acid from myeloid cells by purified colony-stimulating factor. / Ziboh, V. A.; Miller, A. M.; Wu, M. C.; Yunis, Adel A; Jimenez, Joaquin J; Wong, G.

In: Journal of Cellular Physiology, Vol. 113, No. 1, 10.12.1982, p. 67-72.

Research output: Contribution to journalArticle

Ziboh, V. A. ; Miller, A. M. ; Wu, M. C. ; Yunis, Adel A ; Jimenez, Joaquin J ; Wong, G. / Induced release and metabolism of arachidonic acid from myeloid cells by purified colony-stimulating factor. In: Journal of Cellular Physiology. 1982 ; Vol. 113, No. 1. pp. 67-72.
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