Induced rapid phospholipid methylation and arachidonic acid release by dimethyl sulfoxide-treated human promyelocytic leukemic HL-60 cells

Vincent A. Ziboh; Teresa Wong; Ming Chi Wu; Adel A. Yunis

doi:10.1016/0006-291X(86)90762-X

Induced rapid phospholipid methylation and arachidonic acid release by dimethyl sulfoxide-treated human promyelocytic leukemic HL-60 cells

Vincent A. Ziboh, Teresa Wong, Ming Chi Wu, Adel A. Yunis

Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl-ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.

Original language	English (US)
Pages (from-to)	515-522
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	140
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)90762-X
State	Published - Oct 30 1986

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{9e9e7dfe234f493594d642bcb77bd724,

title = "Induced rapid phospholipid methylation and arachidonic acid release by dimethyl sulfoxide-treated human promyelocytic leukemic HL-60 cells",

abstract = "The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl-ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.",

author = "Ziboh, {Vincent A.} and Teresa Wong and Wu, {Ming Chi} and Yunis, {Adel A.}",

note = "Funding Information: ACKNOWLEDGEMENTS We wish to thank Mrs. Roberta Howering for secretarial This study was supported in part by the National Institutes Grants AM-32765, AM31624 and CA-00856, as well as by the Wilderman Memorial Grant for Cancer Research CH-280 from Cancer Society.",

year = "1986",

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doi = "10.1016/0006-291X(86)90762-X",

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T1 - Induced rapid phospholipid methylation and arachidonic acid release by dimethyl sulfoxide-treated human promyelocytic leukemic HL-60 cells

AU - Ziboh, Vincent A.

AU - Wong, Teresa

AU - Wu, Ming Chi

AU - Yunis, Adel A.

N1 - Funding Information: ACKNOWLEDGEMENTS We wish to thank Mrs. Roberta Howering for secretarial This study was supported in part by the National Institutes Grants AM-32765, AM31624 and CA-00856, as well as by the Wilderman Memorial Grant for Cancer Research CH-280 from Cancer Society.

PY - 1986/10/30

Y1 - 1986/10/30

N2 - The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl-ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.

AB - The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl-ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.

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