The incubation of undifferentiated promyelocytic HL-60 cells with DMSO resulted in the rapid transmethylation of phosphatidyl-ethanolamine (PE) into phosphatidylcholine (PC) which was maximal at 60 secs. This rapid generation of PC was followed by a decrease of the methylated phospholipid and the release of arachidonic acid. Thus, the rapid DMSO-induced phospholipid methylation coupled with release of arachidonic acid (precursor for eicosanoids) prior to morphological evidence of cellular differentiation may represent early biochemical events which result in the generation of intracellular chemical signals which may program the promyelocytic cells into a differentiation mode.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and biophysical research communications|
|State||Published - Oct 30 1986|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology