Induced pluripotent stem cells with a mitochondrial dna deletion

Anne B C Cherry, Katelyn E. Gagne, Erin M. McLoughlin, Anna Baccei, Bryan Gorman, Odelya Hartung, Justine D. Miller, Jin Zhang, Rebecca L. Zon, Tan Ince, Ellis J. Neufeld, Paul H. Lerou, Mark D. Fleming, George Q. Daley, Suneet Agarwal

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In congenital mitochondrial DNA (mtDNA) disorders, a mixture of normal and mutated mtDNA (termed heteroplasmy) exists at varying levels in different tissues, which determines the severity and phenotypic expression of disease. Pearson marrow pancreas syndrome (PS) is a congenital bone marrow failure disorder caused by heteroplasmic deletions in mtDNA. The cause of the hematopoietic failure in PS is unknown, and adequate cellular and animal models are lacking. Induced pluripotent stem (iPS) cells are particularly amenable for studying mtDNA disorders, as cytoplasmic genetic material is retained during direct reprogramming. Here, we derive and characterize iPS cells from a patient with PS. Taking advantage of the tendency for heteroplasmy to change with cell passage, we isolated isogenic PS-iPS cells without detectable levels of deleted mtDNA. We found that PS-iPS cells carrying a high burden of deleted mtDNA displayed differences in growth, mitochondrial function, and hematopoietic phenotype when differentiated in vitro, compared to isogenic iPS cells without deleted mtDNA. Our results demonstrate that reprogramming somatic cells from patients with mtDNA disorders can yield pluripotent stem cells with varying burdens of heteroplasmy that might be useful in the study and treatment of mitochondrial diseases.

Original languageEnglish
Pages (from-to)1287-1297
Number of pages11
JournalStem Cells
Volume31
Issue number7
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
Mitochondrial DNA
Mitochondrial Diseases
Pancreas
Pluripotent Stem Cells
Animal Models
Bone Marrow
Phenotype
Growth

Keywords

  • Hematopoiesis
  • Heteroplasmy
  • Human genetics
  • Induced pluripotent stem cells
  • Mitochondrial DNA
  • Pearson's marrow pancreas syndrome

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Cherry, A. B. C., Gagne, K. E., McLoughlin, E. M., Baccei, A., Gorman, B., Hartung, O., ... Agarwal, S. (2013). Induced pluripotent stem cells with a mitochondrial dna deletion. Stem Cells, 31(7), 1287-1297. https://doi.org/10.1002/stem.1354

Induced pluripotent stem cells with a mitochondrial dna deletion. / Cherry, Anne B C; Gagne, Katelyn E.; McLoughlin, Erin M.; Baccei, Anna; Gorman, Bryan; Hartung, Odelya; Miller, Justine D.; Zhang, Jin; Zon, Rebecca L.; Ince, Tan; Neufeld, Ellis J.; Lerou, Paul H.; Fleming, Mark D.; Daley, George Q.; Agarwal, Suneet.

In: Stem Cells, Vol. 31, No. 7, 01.07.2013, p. 1287-1297.

Research output: Contribution to journalArticle

Cherry, ABC, Gagne, KE, McLoughlin, EM, Baccei, A, Gorman, B, Hartung, O, Miller, JD, Zhang, J, Zon, RL, Ince, T, Neufeld, EJ, Lerou, PH, Fleming, MD, Daley, GQ & Agarwal, S 2013, 'Induced pluripotent stem cells with a mitochondrial dna deletion', Stem Cells, vol. 31, no. 7, pp. 1287-1297. https://doi.org/10.1002/stem.1354
Cherry ABC, Gagne KE, McLoughlin EM, Baccei A, Gorman B, Hartung O et al. Induced pluripotent stem cells with a mitochondrial dna deletion. Stem Cells. 2013 Jul 1;31(7):1287-1297. https://doi.org/10.1002/stem.1354
Cherry, Anne B C ; Gagne, Katelyn E. ; McLoughlin, Erin M. ; Baccei, Anna ; Gorman, Bryan ; Hartung, Odelya ; Miller, Justine D. ; Zhang, Jin ; Zon, Rebecca L. ; Ince, Tan ; Neufeld, Ellis J. ; Lerou, Paul H. ; Fleming, Mark D. ; Daley, George Q. ; Agarwal, Suneet. / Induced pluripotent stem cells with a mitochondrial dna deletion. In: Stem Cells. 2013 ; Vol. 31, No. 7. pp. 1287-1297.
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