Induced DNA recombination by Cre recombinase protein transduction

Sunil Joshi, Kahoko Hashimoto, Pandelakis A. Koni

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.

Original languageEnglish (US)
Pages (from-to)48-54
Number of pages7
JournalGenesis
Volume33
Issue number1
DOIs
StatePublished - Jun 17 2002
Externally publishedYes

Fingerprint

Genetic Recombination
Recombinases
DNA
Transgenes
Base Pairing
Proteins
Cell Culture Techniques
Genes
Cre recombinase

Keywords

  • Cre recombinase
  • Gene deletion
  • Induced DNA recombination
  • Protein transduction
  • TAT

ASJC Scopus subject areas

  • Genetics

Cite this

Induced DNA recombination by Cre recombinase protein transduction. / Joshi, Sunil; Hashimoto, Kahoko; Koni, Pandelakis A.

In: Genesis, Vol. 33, No. 1, 17.06.2002, p. 48-54.

Research output: Contribution to journalArticle

Joshi, Sunil ; Hashimoto, Kahoko ; Koni, Pandelakis A. / Induced DNA recombination by Cre recombinase protein transduction. In: Genesis. 2002 ; Vol. 33, No. 1. pp. 48-54.
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