Indirect immunofluorescence on rat bladder transitional epithelium: A test with high specificity for paraneoplastic pemphigus

A. Y. Liu, R. Valenzuela, T. N. Helm, C. Camisa, A. L. Melton, W. F. Bergfeld

Research output: Contribution to journalArticle

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Abstract

Background: Paraneoplastic pemphigus is a blistering disease with specific serum immunoprecipitation findings. Although immunoprecipitation studies allow accurate diagnosis, they are time-consuming, expensive, and not readily available. In contrast, indirect immunofluorescence (IIF) testing of serum on transitional rat bladder epithelium is a simple and inexpensive method available to any immunopathology laboratory. Objective: Our purpose was to determine the specificity of positive IIF on rat bladder epithelium for paraneoplastic pemphigus. Methods: The IIF findings in four index cases of paraneoplastic pemphigus were compared with the findings in 47 patients with a variety of malignant neoplasms and no associated blistering disease as well as 49 patients with vesiculobullous or lichenoid disease but no neoplasia. Results: IIF was negative in all patients with neoplasia and no blistering disease and negative in all but one of the patients with vesiculobullous or lichenoid disease without neoplasia (98.9% specificity). Conclusion: IIF on transitional rat bladder epithelium appears to be a highly specific test for paraneoplastic pemphigus. Because of its simplicity and inexpensiveness, we suggest that IIF be performed on transitional epithelium in any suspected case of paraneoplastic pemphigus.

Original languageEnglish
Pages (from-to)696-699
Number of pages4
JournalJournal of the American Academy of Dermatology
Volume28
Issue number5 I
StatePublished - Jan 1 1993
Externally publishedYes

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Pemphigus
Indirect Fluorescent Antibody Technique
Urinary Bladder
Epithelium
Immunoprecipitation
Neoplasms
Serum

ASJC Scopus subject areas

  • Dermatology

Cite this

Liu, A. Y., Valenzuela, R., Helm, T. N., Camisa, C., Melton, A. L., & Bergfeld, W. F. (1993). Indirect immunofluorescence on rat bladder transitional epithelium: A test with high specificity for paraneoplastic pemphigus. Journal of the American Academy of Dermatology, 28(5 I), 696-699.

Indirect immunofluorescence on rat bladder transitional epithelium : A test with high specificity for paraneoplastic pemphigus. / Liu, A. Y.; Valenzuela, R.; Helm, T. N.; Camisa, C.; Melton, A. L.; Bergfeld, W. F.

In: Journal of the American Academy of Dermatology, Vol. 28, No. 5 I, 01.01.1993, p. 696-699.

Research output: Contribution to journalArticle

Liu, A. Y. ; Valenzuela, R. ; Helm, T. N. ; Camisa, C. ; Melton, A. L. ; Bergfeld, W. F. / Indirect immunofluorescence on rat bladder transitional epithelium : A test with high specificity for paraneoplastic pemphigus. In: Journal of the American Academy of Dermatology. 1993 ; Vol. 28, No. 5 I. pp. 696-699.
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