Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels

Xiao Wang, Carlos T Moraes

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Although mitochondrial respiration is decreased in most cancer cells, the role of this decrease in carcinogenesis and cancer progression is still unclear. To better understand this phenomenon, instead of further inhibiting mitochondrial function, we induced mitochondrial biogenesis in transformed cells by activating the peroxisome proliferator-activated receptors (PPARs)/peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α) pathways. This was achieved by treating the cells with bezafibrate, a PPARs panagonist that also enhances PGC-1α expression. We confirmed that bezafibrate treatment led to increased mitochondrial proteins and enzyme functions. We found that cells with increased mitochondrial biogenesis had decreased growth rates in glucose-containing medium. In addition, they became less invasive, which was directly linked to the reduced lactate levels. Surprisingly, even though bezafibrate-treated cells had higher levels of mitochondrial markers, total respiration was not significantly altered. However, respiratory coupling, and ATP levels were. Our data show that by increasing the efficiency of the mitochondrial oxidative phosphorylation system, cancer progression is hampered by decreases in cell proliferation and invasiveness.

Original languageEnglish
Pages (from-to)399-409
Number of pages11
JournalMolecular Oncology
Volume5
Issue number5
DOIs
StatePublished - Oct 1 2011

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Organelle Biogenesis
Bezafibrate
Carcinogenesis
Peroxisome Proliferator-Activated Receptors
Respiration
Neoplasms
PPAR gamma
Mitochondrial Proteins
Oxidative Phosphorylation
Lactic Acid
Adenosine Triphosphate
Cell Proliferation
Glucose
Enzymes
Growth

Keywords

  • Bezafibrate
  • Invasion
  • Mitochondria
  • PPAR
  • Tumorigenesis

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Medicine

Cite this

Increases in mitochondrial biogenesis impair carcinogenesis at multiple levels. / Wang, Xiao; Moraes, Carlos T.

In: Molecular Oncology, Vol. 5, No. 5, 01.10.2011, p. 399-409.

Research output: Contribution to journalArticle

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