TY - JOUR
T1 - Increased splenic transplantable colony-forming units related to increased radioresistance after rauscher leukemia virus infection of the SJL/J mouse
AU - Markoe, Arnold M.
AU - Okunewick, James P.
AU - Erhard, Penny
AU - Phillips, Eleanore
N1 - Funding Information:
1 Received August 10, 1972; accepted October 6, 1972. 2 Supported in part by Public Health Service research grant 1P02 CA10438-05 from the National Cancer Institute, by the Atomic Energy Commission contract AT(11-1)-3097, and grant HRSF N-32 of the United Fund of Pittsburgh. One of us (A.M.M.) was partially supported by Public Health Service training grant RL 00063.
PY - 1973/2
Y1 - 1973/2
N2 - Using the exogenous colony-forming unit (CFU) assay of Till and McCulloch, we studied changes in splenic CFU content as a function of time after Rauscher leukemia virus (RLV) infection of the SJL/J mouse, and we related splenic CFU content to previously reported changes in radiosensitivity after infection. Total splenic CFU content [colony-forming potential (CFP)/spleen] first increased after the 6th day of infection, correlating with the appearance of splenomegaly and of radioresistance. CFP/spleen continued to increase until day 22 after infection, reaching 16 times normal levels. This increase was positively correlated with greater radioresistance. Beyond the 22d day of infection, CFP/spleen fell somewhat but was never `9 times normal values. However, calculations based on existing information regarding spleen cell radiation survival and turnover indicated that this level of splenic CFU was sufficient to provide radioprotection at the level of X-ray exposure used. We conclude, from these studies, that RLV infection increased the number of splenic hematopoietic stem cells of the SJL/J mouse and this increase resulted in the greater radioresistance.
AB - Using the exogenous colony-forming unit (CFU) assay of Till and McCulloch, we studied changes in splenic CFU content as a function of time after Rauscher leukemia virus (RLV) infection of the SJL/J mouse, and we related splenic CFU content to previously reported changes in radiosensitivity after infection. Total splenic CFU content [colony-forming potential (CFP)/spleen] first increased after the 6th day of infection, correlating with the appearance of splenomegaly and of radioresistance. CFP/spleen continued to increase until day 22 after infection, reaching 16 times normal levels. This increase was positively correlated with greater radioresistance. Beyond the 22d day of infection, CFP/spleen fell somewhat but was never `9 times normal values. However, calculations based on existing information regarding spleen cell radiation survival and turnover indicated that this level of splenic CFU was sufficient to provide radioprotection at the level of X-ray exposure used. We conclude, from these studies, that RLV infection increased the number of splenic hematopoietic stem cells of the SJL/J mouse and this increase resulted in the greater radioresistance.
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U2 - 10.1093/jnci/50.2.449
DO - 10.1093/jnci/50.2.449
M3 - Article
C2 - 4702117
AN - SCOPUS:0015581042
VL - 50
SP - 449
EP - 455
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 2
ER -