Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP

V. Fontana, Wenche Jy, E. R. Ahn, P. Dudkiewicz, L. L. Horstman, R. Duncan, Yeon Ahn

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background: Splenectomy is frequently employed for therapeutic and diagnostic purposes in various clinical disorders. However its long-term safety is not well elucidated. Although risk of infection by encapsulated organisms is widely recognized, less well-known are risks of thrombosis and cardiovascular disease. Methods: We investigated levels of cell-derived microparticles (C-MP) in 23 splenectomized ITP (ITP-S) and 53 unsplenectomized ITP patients (ITP-nS). Assay of C-MP derived from platelets (PMP), leukocytes (LMP), red cells (RMP) and endothelial cells (EMP) were performed by flow cytometry. Coagulation parameters included PT, aPTT and activities of FVIII, IX and XI. Results of all measures were compared between the two groups, ITP-S vs ITP-nS. Results: Levels of all C-MP were higher in ITP-S than ITP-nS but only RMP and LMP reached statistical significance (p = 0.0035 and p < 0.0001, respectively). The aPTT was significantly shorter in ITP-S (p = 0.029). Interestingly, correlation analysis revealed that RMP, but not other C-MP, were associated with shortening of aPTT (p = 0.024) as well as with increased activities of factors VIII (p = 0.023), IX (p = 0.021) and XI (p = 0.0089). Conclusions: RMP and LMP were significantly elevated in splenectomized compared to non-splenectomized ITP patients. This suggests that the spleen functions to clear procoagulant C-MP, and that elevation of C-MP might contribute to increased risk of thrombosis, progression of atherosclerosis and cardiovascular disease following splenectomy.

Original languageEnglish
Pages (from-to)599-603
Number of pages5
JournalThrombosis Research
Volume122
Issue number5
DOIs
StatePublished - Mar 11 2008

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Cell-Derived Microparticles
Inosine Triphosphate
Splenectomy
Thrombosis
Cardiovascular Diseases
Factor VIII
Atherosclerosis
Flow Cytometry
Leukocytes
Blood Platelets
Spleen
Endothelial Cells

Keywords

  • Cardiovascular disease
  • ITP
  • Microparticles
  • Red cell microparticles
  • Splenectomy

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP. / Fontana, V.; Jy, Wenche; Ahn, E. R.; Dudkiewicz, P.; Horstman, L. L.; Duncan, R.; Ahn, Yeon.

In: Thrombosis Research, Vol. 122, No. 5, 11.03.2008, p. 599-603.

Research output: Contribution to journalArticle

Fontana, V, Jy, W, Ahn, ER, Dudkiewicz, P, Horstman, LL, Duncan, R & Ahn, Y 2008, 'Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP', Thrombosis Research, vol. 122, no. 5, pp. 599-603. https://doi.org/10.1016/j.thromres.2007.12.022
Fontana, V. ; Jy, Wenche ; Ahn, E. R. ; Dudkiewicz, P. ; Horstman, L. L. ; Duncan, R. ; Ahn, Yeon. / Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP. In: Thrombosis Research. 2008 ; Vol. 122, No. 5. pp. 599-603.
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abstract = "Background: Splenectomy is frequently employed for therapeutic and diagnostic purposes in various clinical disorders. However its long-term safety is not well elucidated. Although risk of infection by encapsulated organisms is widely recognized, less well-known are risks of thrombosis and cardiovascular disease. Methods: We investigated levels of cell-derived microparticles (C-MP) in 23 splenectomized ITP (ITP-S) and 53 unsplenectomized ITP patients (ITP-nS). Assay of C-MP derived from platelets (PMP), leukocytes (LMP), red cells (RMP) and endothelial cells (EMP) were performed by flow cytometry. Coagulation parameters included PT, aPTT and activities of FVIII, IX and XI. Results of all measures were compared between the two groups, ITP-S vs ITP-nS. Results: Levels of all C-MP were higher in ITP-S than ITP-nS but only RMP and LMP reached statistical significance (p = 0.0035 and p < 0.0001, respectively). The aPTT was significantly shorter in ITP-S (p = 0.029). Interestingly, correlation analysis revealed that RMP, but not other C-MP, were associated with shortening of aPTT (p = 0.024) as well as with increased activities of factors VIII (p = 0.023), IX (p = 0.021) and XI (p = 0.0089). Conclusions: RMP and LMP were significantly elevated in splenectomized compared to non-splenectomized ITP patients. This suggests that the spleen functions to clear procoagulant C-MP, and that elevation of C-MP might contribute to increased risk of thrombosis, progression of atherosclerosis and cardiovascular disease following splenectomy.",
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T1 - Increased procoagulant cell-derived microparticles (C-MP) in splenectomized patients with ITP

AU - Fontana, V.

AU - Jy, Wenche

AU - Ahn, E. R.

AU - Dudkiewicz, P.

AU - Horstman, L. L.

AU - Duncan, R.

AU - Ahn, Yeon

PY - 2008/3/11

Y1 - 2008/3/11

N2 - Background: Splenectomy is frequently employed for therapeutic and diagnostic purposes in various clinical disorders. However its long-term safety is not well elucidated. Although risk of infection by encapsulated organisms is widely recognized, less well-known are risks of thrombosis and cardiovascular disease. Methods: We investigated levels of cell-derived microparticles (C-MP) in 23 splenectomized ITP (ITP-S) and 53 unsplenectomized ITP patients (ITP-nS). Assay of C-MP derived from platelets (PMP), leukocytes (LMP), red cells (RMP) and endothelial cells (EMP) were performed by flow cytometry. Coagulation parameters included PT, aPTT and activities of FVIII, IX and XI. Results of all measures were compared between the two groups, ITP-S vs ITP-nS. Results: Levels of all C-MP were higher in ITP-S than ITP-nS but only RMP and LMP reached statistical significance (p = 0.0035 and p < 0.0001, respectively). The aPTT was significantly shorter in ITP-S (p = 0.029). Interestingly, correlation analysis revealed that RMP, but not other C-MP, were associated with shortening of aPTT (p = 0.024) as well as with increased activities of factors VIII (p = 0.023), IX (p = 0.021) and XI (p = 0.0089). Conclusions: RMP and LMP were significantly elevated in splenectomized compared to non-splenectomized ITP patients. This suggests that the spleen functions to clear procoagulant C-MP, and that elevation of C-MP might contribute to increased risk of thrombosis, progression of atherosclerosis and cardiovascular disease following splenectomy.

AB - Background: Splenectomy is frequently employed for therapeutic and diagnostic purposes in various clinical disorders. However its long-term safety is not well elucidated. Although risk of infection by encapsulated organisms is widely recognized, less well-known are risks of thrombosis and cardiovascular disease. Methods: We investigated levels of cell-derived microparticles (C-MP) in 23 splenectomized ITP (ITP-S) and 53 unsplenectomized ITP patients (ITP-nS). Assay of C-MP derived from platelets (PMP), leukocytes (LMP), red cells (RMP) and endothelial cells (EMP) were performed by flow cytometry. Coagulation parameters included PT, aPTT and activities of FVIII, IX and XI. Results of all measures were compared between the two groups, ITP-S vs ITP-nS. Results: Levels of all C-MP were higher in ITP-S than ITP-nS but only RMP and LMP reached statistical significance (p = 0.0035 and p < 0.0001, respectively). The aPTT was significantly shorter in ITP-S (p = 0.029). Interestingly, correlation analysis revealed that RMP, but not other C-MP, were associated with shortening of aPTT (p = 0.024) as well as with increased activities of factors VIII (p = 0.023), IX (p = 0.021) and XI (p = 0.0089). Conclusions: RMP and LMP were significantly elevated in splenectomized compared to non-splenectomized ITP patients. This suggests that the spleen functions to clear procoagulant C-MP, and that elevation of C-MP might contribute to increased risk of thrombosis, progression of atherosclerosis and cardiovascular disease following splenectomy.

KW - Cardiovascular disease

KW - ITP

KW - Microparticles

KW - Red cell microparticles

KW - Splenectomy

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DO - 10.1016/j.thromres.2007.12.022

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