Increased NADPH-diaphorase reactivity and cytokine expression in dorsal root ganglia in acquired immunodeficiency syndrome

I. Nagano, P. Shapshak, M. Yoshioka, K. Xin, S. Nakamura, Walter G Bradley

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Abstract

We studied lumbosacral dorsal root ganglia (DRGs) from 10 patients with acquired immunodeficiency syndrome (AIDS) and five controls using immunocytochemistry, in situ hybridization and NADPH-diaphorase (NADPHd) histochemistry. Human immunodeficiency virus (HIV)-1 RNA was detected in five AIDS cases, and HIV-1 p24 antigen was found in four of these patients. The densities of nodules of Nageotte (nN), macrophages and major histocompatibility complex-class II-positive cells were significantly increased in the DRGs of AIDS patients compared to controls. Cytomegalovirus antigen was observed in the DRGs of four AIDS cases and one control, but without its presence being related to neuronal degeneration. Furthermore, we detected tumor necrosis factor, interferon-γ, interleukin (IL)-1β, and IL-6 in the DRGs from AIDS patients. Using NADPHd histochemistry, we showed that the number of NADPHd-positive neurons was significantly increased in the DRGs of AIDS patients compared to controls, implying upregulation of nitric-oxide (NO) production in AIDS DRGs. Generally, there were increased numbers of nN in DRGs which contained more NADPHd-positive neurons. Additionally, immunoreactivity for an inducible form of NO synthase was detected in interstitial cells in AIDS DRGs. These results suggest that reactive inflammation, including the production of cytokines, occurs in the DRGs of AIDS patients and that excessive production of NO may be related to neuronal degeneration in AIDS DRGs.

Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalJournal of the Neurological Sciences
Volume136
Issue number1-2
DOIs
StatePublished - Apr 2 1996

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NADPH Dehydrogenase
Spinal Ganglia
Acquired Immunodeficiency Syndrome
Cytokines
HIV-1
Nitric Oxide
Neurons
Antigens
Nitric Oxide Synthase Type II
Major Histocompatibility Complex
Cytomegalovirus
Interleukin-1
Interferons
In Situ Hybridization
Interleukin-6
Up-Regulation
Tumor Necrosis Factor-alpha
Immunohistochemistry
Macrophages
RNA

Keywords

  • AIDS
  • Cytokine
  • Dorsal root ganglion
  • NADPH diaphorase
  • Nitric oxide
  • Sensory neuron

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Developmental Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

Increased NADPH-diaphorase reactivity and cytokine expression in dorsal root ganglia in acquired immunodeficiency syndrome. / Nagano, I.; Shapshak, P.; Yoshioka, M.; Xin, K.; Nakamura, S.; Bradley, Walter G.

In: Journal of the Neurological Sciences, Vol. 136, No. 1-2, 02.04.1996, p. 117-128.

Research output: Contribution to journalArticle

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