TY - JOUR
T1 - Increased NADPH-diaphorase reactivity and cytokine expression in dorsal root ganglia in acquired immunodeficiency syndrome
AU - Nagano, I.
AU - Shapshak, P.
AU - Yoshioka, M.
AU - Xin, K.
AU - Nakamura, S.
AU - Bradley, W. G.
N1 - Funding Information:
The authors thank Dr. C.K. Petit0 for helpful discussions, and Mr. T.H. Davis and Ms. R.V. Stewart for technical assistance.D r. R.K. Wright and the staff of the Broward County Medical Examiner’s Office (Fort Lauderdale, FL), and the staff of the Departmento f Pathology, University of Miami School of Medicine (Miami, FL) are thanked for providing tissue specimens. The IL-6 monoclonal antibody was generously supplied by Genetics Insti-tute, Inc. (Cambridge, MA). This work was supportedb y NIH grants NS 3 1488, DA 04787 and DA 07909.
PY - 1996
Y1 - 1996
N2 - We studied lumbosacral dorsal root ganglia (DRGs) from 10 patients with acquired immunodeficiency syndrome (AIDS) and five controls using immunocytochemistry, in situ hybridization and NADPH-diaphorase (NADPHd) histochemistry. Human immunodeficiency virus (HIV)-1 RNA was detected in five AIDS cases, and HIV-1 p24 antigen was found in four of these patients. The densities of nodules of Nageotte (nN), macrophages and major histocompatibility complex-class II-positive cells were significantly increased in the DRGs of AIDS patients compared to controls. Cytomegalovirus antigen was observed in the DRGs of four AIDS cases and one control, but without its presence being related to neuronal degeneration. Furthermore, we detected tumor necrosis factor, interferon-γ, interleukin (IL)-1β, and IL-6 in the DRGs from AIDS patients. Using NADPHd histochemistry, we showed that the number of NADPHd-positive neurons was significantly increased in the DRGs of AIDS patients compared to controls, implying upregulation of nitric-oxide (NO) production in AIDS DRGs. Generally, there were increased numbers of nN in DRGs which contained more NADPHd-positive neurons. Additionally, immunoreactivity for an inducible form of NO synthase was detected in interstitial cells in AIDS DRGs. These results suggest that reactive inflammation, including the production of cytokines, occurs in the DRGs of AIDS patients and that excessive production of NO may be related to neuronal degeneration in AIDS DRGs.
AB - We studied lumbosacral dorsal root ganglia (DRGs) from 10 patients with acquired immunodeficiency syndrome (AIDS) and five controls using immunocytochemistry, in situ hybridization and NADPH-diaphorase (NADPHd) histochemistry. Human immunodeficiency virus (HIV)-1 RNA was detected in five AIDS cases, and HIV-1 p24 antigen was found in four of these patients. The densities of nodules of Nageotte (nN), macrophages and major histocompatibility complex-class II-positive cells were significantly increased in the DRGs of AIDS patients compared to controls. Cytomegalovirus antigen was observed in the DRGs of four AIDS cases and one control, but without its presence being related to neuronal degeneration. Furthermore, we detected tumor necrosis factor, interferon-γ, interleukin (IL)-1β, and IL-6 in the DRGs from AIDS patients. Using NADPHd histochemistry, we showed that the number of NADPHd-positive neurons was significantly increased in the DRGs of AIDS patients compared to controls, implying upregulation of nitric-oxide (NO) production in AIDS DRGs. Generally, there were increased numbers of nN in DRGs which contained more NADPHd-positive neurons. Additionally, immunoreactivity for an inducible form of NO synthase was detected in interstitial cells in AIDS DRGs. These results suggest that reactive inflammation, including the production of cytokines, occurs in the DRGs of AIDS patients and that excessive production of NO may be related to neuronal degeneration in AIDS DRGs.
KW - AIDS
KW - Cytokine
KW - Dorsal root ganglion
KW - NADPH diaphorase
KW - Nitric oxide
KW - Sensory neuron
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U2 - 10.1016/0022-510X(95)00317-U
DO - 10.1016/0022-510X(95)00317-U
M3 - Article
C2 - 8815158
AN - SCOPUS:0029877341
VL - 136
SP - 117
EP - 128
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 1-2
ER -