Abstract
Methionine is converted by the transmethylation/transsulfuration pathway to homocysteine which may exert atherogenic effects by several mechanisms, including lipid peroxidation. Therefore, the excessive dietary methionine may induce the development of atherosclerosis. To test this hypothesis, plasma and aortic thiobarbituric acid reactive substances (TBARS), as well as activities of aortic and erythrocyte superoxide dismutase (SOD), catalase and selenium-dependent glutathione peroxidase (GPX) were measured in rabbits fed a diet enriched with 0.3% methionine for 6 or 9 months. Histological examinations of aortas also were performed. Feeding rabbits a methionine-enriched diet for 6 or 9 months resulted in significant increases in plasma and aortic TBARS levels and aortic antioxidant enzyme activities. However, a decrease in plasma antioxidant activity (AOA) was observed. In erythrocytes, SOD activity increased, catalase remained normal and GPX decreased in the treated animals. Histological examination of aortas showed typical atherosclerotic changes, such as intimal thickening, deposition of cholesterol, and calcification in methionine-fed rabbits. These results confirm that high-methionine diet may induce atherosclerosis in rabbits and indicate disturbances in lipid peroxidation and antioxidant processes as possible mechanisms of its atherogenic influence.
| Original language | English |
|---|---|
| Pages (from-to) | 217-224 |
| Number of pages | 8 |
| Journal | Atherosclerosis |
| Volume | 115 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1995 |
| Externally published | Yes |
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Keywords
- Antioxidative enzymes
- Atherosclerosis
- Methionine
- Oxidative stress
- Rabbits
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits. / Toborek, Michal J; Kopieczna-Grzebieniak, Ewa; Drózdz, Marian; Wieczorek, Marian.
In: Atherosclerosis, Vol. 115, No. 2, 01.01.1995, p. 217-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits
AU - Toborek, Michal J
AU - Kopieczna-Grzebieniak, Ewa
AU - Drózdz, Marian
AU - Wieczorek, Marian
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Methionine is converted by the transmethylation/transsulfuration pathway to homocysteine which may exert atherogenic effects by several mechanisms, including lipid peroxidation. Therefore, the excessive dietary methionine may induce the development of atherosclerosis. To test this hypothesis, plasma and aortic thiobarbituric acid reactive substances (TBARS), as well as activities of aortic and erythrocyte superoxide dismutase (SOD), catalase and selenium-dependent glutathione peroxidase (GPX) were measured in rabbits fed a diet enriched with 0.3% methionine for 6 or 9 months. Histological examinations of aortas also were performed. Feeding rabbits a methionine-enriched diet for 6 or 9 months resulted in significant increases in plasma and aortic TBARS levels and aortic antioxidant enzyme activities. However, a decrease in plasma antioxidant activity (AOA) was observed. In erythrocytes, SOD activity increased, catalase remained normal and GPX decreased in the treated animals. Histological examination of aortas showed typical atherosclerotic changes, such as intimal thickening, deposition of cholesterol, and calcification in methionine-fed rabbits. These results confirm that high-methionine diet may induce atherosclerosis in rabbits and indicate disturbances in lipid peroxidation and antioxidant processes as possible mechanisms of its atherogenic influence.
AB - Methionine is converted by the transmethylation/transsulfuration pathway to homocysteine which may exert atherogenic effects by several mechanisms, including lipid peroxidation. Therefore, the excessive dietary methionine may induce the development of atherosclerosis. To test this hypothesis, plasma and aortic thiobarbituric acid reactive substances (TBARS), as well as activities of aortic and erythrocyte superoxide dismutase (SOD), catalase and selenium-dependent glutathione peroxidase (GPX) were measured in rabbits fed a diet enriched with 0.3% methionine for 6 or 9 months. Histological examinations of aortas also were performed. Feeding rabbits a methionine-enriched diet for 6 or 9 months resulted in significant increases in plasma and aortic TBARS levels and aortic antioxidant enzyme activities. However, a decrease in plasma antioxidant activity (AOA) was observed. In erythrocytes, SOD activity increased, catalase remained normal and GPX decreased in the treated animals. Histological examination of aortas showed typical atherosclerotic changes, such as intimal thickening, deposition of cholesterol, and calcification in methionine-fed rabbits. These results confirm that high-methionine diet may induce atherosclerosis in rabbits and indicate disturbances in lipid peroxidation and antioxidant processes as possible mechanisms of its atherogenic influence.
KW - Antioxidative enzymes
KW - Atherosclerosis
KW - Methionine
KW - Oxidative stress
KW - Rabbits
UR - http://www.scopus.com/inward/record.url?scp=0029051274&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029051274&partnerID=8YFLogxK
U2 - 10.1016/0021-9150(94)05516-L
DO - 10.1016/0021-9150(94)05516-L
M3 - Article
C2 - 7661880
AN - SCOPUS:0029051274
VL - 115
SP - 217
EP - 224
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
IS - 2
ER -